Abstract

The purpose of this core is to provide morphology support services for project participants using transmission electron microscopy, fluorescence light microscopy, immunofluorescence, immunocytochemistry, photography, cryoultramicrotomy, electron probe analysis, morphometry, autoradiography, quantitative confocal microscopy, and in situ hybridization. The core houses a JEOL 100 CX Electron Microscope, a BioRad 2000 confocal microscope and a Metamorph based image collection and processing. The technical staff of the core supplies the assistance needed to each investigator to use these specializes tools and techniques.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL019737-29
Application #
6994410
Study Section
Project Start
Project End
Budget Start
2004-12-01
Budget End
2005-11-30
Support Year
29
Fiscal Year
2005
Total Cost
$260,015
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Hawkins, Arie; Guttentag, Susan H; Deterding, Robin et al. (2015) A non-BRICHOS SFTPC mutant (SP-CI73T) linked to interstitial lung disease promotes a late block in macroautophagy disrupting cellular proteostasis and mitophagy. Am J Physiol Lung Cell Mol Physiol 308:L33-47
Chowdhury, Ibrul; Fisher, Aron B; Christofidou-Solomidou, Melpo et al. (2014) Keratinocyte growth factor and glucocorticoid induction of human peroxiredoxin 6 gene expression occur by independent mechanisms that are synergistic. Antioxid Redox Signal 20:391-402
Roszell, Blair R; Tao, Jian-Qin; Yu, Kevin J et al. (2013) Pulmonary abnormalities in animal models due to Niemann-Pick type C1 (NPC1) or C2 (NPC2) disease. PLoS One 8:e67084
Roszell, Blair R; Tao, Jian-Qin; Yu, Kevin J et al. (2012) Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung. Am J Physiol Lung Cell Mol Physiol 302:L919-32
Rahaman, Hamidur; Zhou, Suiping; Dodia, Chandra et al. (2012) Increased phospholipase A2 activity with phosphorylation of peroxiredoxin 6 requires a conformational change in the protein. Biochemistry 51:5521-30
Weibel, Ginny L; Joshi, Michelle R; Jerome, W Gray et al. (2012) Cytoskeleton disruption in J774 macrophages: consequences for lipid droplet formation and cholesterol flux. Biochim Biophys Acta 1821:464-72
Maguire, Jean Ann; Mulugeta, Surafel; Beers, Michael F (2012) Multiple ways to die: delineation of the unfolded protein response and apoptosis induced by Surfactant Protein C BRICHOS mutants. Int J Biochem Cell Biol 44:101-12
Atochina-Vasserman, Elena N; Bates, Sandra R; Zhang, Peggy et al. (2011) Early alveolar epithelial dysfunction promotes lung inflammation in a mouse model of Hermansky-Pudlak syndrome. Am J Respir Crit Care Med 184:449-58
Zhong, Qian; Zhou, Beiyun; Ann, David K et al. (2011) Role of endoplasmic reticulum stress in epithelial-mesenchymal transition of alveolar epithelial cells: effects of misfolded surfactant protein. Am J Respir Cell Mol Biol 45:498-509
Beers, Michael F; Morrisey, Edward E (2011) The three R's of lung health and disease: repair, remodeling, and regeneration. J Clin Invest 121:2065-73

Showing the most recent 10 out of 234 publications