Abstract

It is the objective of this laboratory to provide plasma lipoproteins and purified apolipoproteins isolated according to carefully standardized procedures for use by the individual projects within the program. This core facility is central to the Program Project and every member utilizes it. This core has three main purposes which are; I. Lipoprotein Production Isolation and purification of human lipoproteins and related products II. Apolipoprotein Production Isolation of human apolipoproteins including apo A-I, A-II and apo C-I. C- II and C-III. III. Characterization of Lipoproteins and Apolipoproteins Using the Following Techniques: (a) Agarose gel electrophoresis of lipoproteins (b) SDS-PAGE of apolipoproteins (c) Electron microscopy of native and reconstituted lipid-protein complexes. The benefits arising from the centralized service provided by the Core Laboratory include: 1) uniformity of materials and techniques; 2) optimal use of space, supplies and equipment, especially centrifuges.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL022633-26
Application #
6642920
Study Section
Project Start
2002-07-01
Project End
2003-06-30
Budget Start
Budget End
Support Year
26
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
Mcp Hahnemann University
Department
Type
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19102

  Publications

Cuchel, Marina; Raper, Anna C; Conlon, Donna M et al. (2017) A novel approach to measuring macrophage-specific reverse cholesterol transport in vivo in humans. J Lipid Res 58:752-762
Nagao, Kohjiro; Hata, Mami; Tanaka, Kento et al. (2014) The roles of C-terminal helices of human apolipoprotein A-I in formation of high-density lipoprotein particles. Biochim Biophys Acta 1841:80-7
Weibel, Ginny L; Drazul-Schrader, Denise; Shivers, Debra K et al. (2014) Importance of evaluating cell cholesterol influx with efflux in determining the impact of human serum on cholesterol metabolism and atherosclerosis. Arterioscler Thromb Vasc Biol 34:17-25
Phillips, Michael C (2014) Molecular mechanisms of cellular cholesterol efflux. J Biol Chem 289:24020-9
Yang, Yanbo; Kuwano, Takashi; Lagor, William R et al. (2014) Lipidomic analyses of female mice lacking hepatic lipase and endothelial lipase indicate selective modulation of plasma lipid species. Lipids 49:505-15
Lagor, William R; Fields, David W; Bauer, Robert C et al. (2014) Genetic manipulation of the ApoF/Stat2 locus supports an important role for type I interferon signaling in atherosclerosis. Atherosclerosis 233:234-41
Chetty, Palaniappan Sevugan; Nguyen, David; Nickel, Margaret et al. (2013) Comparison of apoA-I helical structure and stability in discoidal and spherical HDL particles by HX and mass spectrometry. J Lipid Res 54:1589-97
Nguyen, David; Nickel, Margaret; Mizuguchi, Chiharu et al. (2013) Interactions of apolipoprotein A-I with high-density lipoprotein particles. Biochemistry 52:1963-72
Alexander, Eric T; Phillips, Michael C (2013) Influence of apolipoprotein A-I and apolipoprotein A-II availability on nascent HDL heterogeneity. J Lipid Res 54:3464-70
Patel, Parin J; Khera, Amit V; Wilensky, Robert L et al. (2013) Anti-oxidative and cholesterol efflux capacities of high-density lipoprotein are reduced in ischaemic cardiomyopathy. Eur J Heart Fail 15:1215-9

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