It is the objective of this laboratory to provide plasma lipoproteins and purified apolipoproteins isolated according to carefully standardized procedures for use by the individual projects within the program. This core facility is central to the Program Project and every member utilizes it. This core has three main purposes which are; I. Lipoprotein Production Isolation and purification of human lipoproteins and related products II. Apolipoprotein Production Isolation of human apolipoproteins including apo A-I, A-II and apo C-I. C- II and C-III. III. Characterization of Lipoproteins and Apolipoproteins Using the Following Techniques: (a) Agarose gel electrophoresis of lipoproteins (b) SDS-PAGE of apolipoproteins (c) Electron microscopy of native and reconstituted lipid-protein complexes. The benefits arising from the centralized service provided by the Core Laboratory include: 1) uniformity of materials and techniques; 2) optimal use of space, supplies and equipment, especially centrifuges.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL022633-26
Application #
6642920
Study Section
Project Start
2002-07-01
Project End
2003-06-30
Budget Start
Budget End
Support Year
26
Fiscal Year
2002
Total Cost
Indirect Cost
Name
Mcp Hahnemann University
Department
Type
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19102
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