Abstract

The objective of the tissue culture laboratory is to support the investigators of the Program Project who use cells models. A centralized Tissue Culture Core facility has several advantages: 1) it conserves space, eliminates the need for redundant equipment and allows for the efficient acquisition of supplies; 2) it ensures standardization of procedures and cell lines; 3) it facilitates the introduction of new cell models into the program; 4) serves as a training facility. The Tissue Culture Core facility is an integral part of all the projects in the current program.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL022633-27
Application #
6740693
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2003-07-01
Project End
2008-06-30
Budget Start
2003-07-01
Budget End
2008-06-30
Support Year
27
Fiscal Year
2003
Total Cost
$103,779
Indirect Cost

  Institution

Name
Children's Hospital of Philadelphia
Department
Type
DUNS #
073757627
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Cuchel, Marina; Raper, Anna C; Conlon, Donna M et al. (2017) A novel approach to measuring macrophage-specific reverse cholesterol transport in vivo in humans. J Lipid Res 58:752-762
Nagao, Kohjiro; Hata, Mami; Tanaka, Kento et al. (2014) The roles of C-terminal helices of human apolipoprotein A-I in formation of high-density lipoprotein particles. Biochim Biophys Acta 1841:80-7
Weibel, Ginny L; Drazul-Schrader, Denise; Shivers, Debra K et al. (2014) Importance of evaluating cell cholesterol influx with efflux in determining the impact of human serum on cholesterol metabolism and atherosclerosis. Arterioscler Thromb Vasc Biol 34:17-25
Phillips, Michael C (2014) Molecular mechanisms of cellular cholesterol efflux. J Biol Chem 289:24020-9
Yang, Yanbo; Kuwano, Takashi; Lagor, William R et al. (2014) Lipidomic analyses of female mice lacking hepatic lipase and endothelial lipase indicate selective modulation of plasma lipid species. Lipids 49:505-15
Lagor, William R; Fields, David W; Bauer, Robert C et al. (2014) Genetic manipulation of the ApoF/Stat2 locus supports an important role for type I interferon signaling in atherosclerosis. Atherosclerosis 233:234-41
Patel, Parin J; Khera, Amit V; Wilensky, Robert L et al. (2013) Anti-oxidative and cholesterol efflux capacities of high-density lipoprotein are reduced in ischaemic cardiomyopathy. Eur J Heart Fail 15:1215-9
Phillips, Michael C (2013) New insights into the determination of HDL structure by apolipoproteins: Thematic review series: high density lipoprotein structure, function, and metabolism. J Lipid Res 54:2034-48
Lund-Katz, Sissel; Lyssenko, Nicholas N; Nickel, Margaret et al. (2013) Mechanisms responsible for the compositional heterogeneity of nascent high density lipoprotein. J Biol Chem 288:23150-60
Lyssenko, Nicholas N; Nickel, Margaret; Tang, Chongren et al. (2013) Factors controlling nascent high-density lipoprotein particle heterogeneity: ATP-binding cassette transporter A1 activity and cell lipid and apolipoprotein AI availability. FASEB J 27:2880-92

Showing the most recent 10 out of 336 publications