The Microscopy and Image Analysis Core is a well-equipped facility that serves all investigators in the Program Project and consolidates all tissue preparation, microscopy and image analysis in the same laboratory under the direction of staff with expertise in all aspects of our morphology and microscopy program. The laboratory provides investigators and their associates with the expertise, equipment, supplies, and when necessary, the training needed for their morphological research. Associated with the relocation of our laboratories to the Laurel Heights Campus and the closure of the C.V.R.I. Core Microscopy Laboratory on the Parnassus Campus. several major changes in the structure of the Core Laboratory have taken place since the last renewal of Program Project Grant. These changes include the relocation of our equipment, including the electron microscope, cryostat, microtome, immunofluorescence microscope, image analysis system and fully equipped photographic darkroom, to the Laurel Heights Campus, consolidation of all our microscopy and image analysis operations in a single laboratory , and designation of a new Core Director. The restructured Core has been fully operation in our new and expanded facilities since January 1998. The restructuring has led to greater operational and financial efficiencies that are reflected in the quality of the recent publications and preliminary data included in this application. The restructured Core retains the advantages of the previous shared laboratory, namely the fact that specialized equipment is readily available. The consolidation of all our laboratory space, including a fully equipped Microscopy Laboratory, enhances the ability of the Core staff to provide individual attention to the particular problems involved in individual projects. The organization of the Microscopy and Image Analysis Core in the current application reflects these new realities.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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University of California San Francisco
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Barrette, Anne Marie; Roberts, Jessica K; Chapin, Cheryl et al. (2016) Antiinflammatory Effects of Budesonide in Human Fetal Lung. Am J Respir Cell Mol Biol 55:623-632
Danhaive, Olivier; Chapin, Cheryl; Horneman, Hart et al. (2015) Surface film formation in vitro by infant and therapeutic surfactants: role of surfactant protein B. Pediatr Res 77:340-6
Vanderbilt, Jeff N; Gonzalez, Robert F; Allen, Lennell et al. (2015) High-efficiency type II cell-enhanced green fluorescent protein expression facilitates cellular identification, tracking, and isolation. Am J Respir Cell Mol Biol 53:14-21
Gonzales, Linda W; Gonzalez, Robert; Barrette, Anne Marie et al. (2015) Expression of Carcinoembryonic Cell Adhesion Molecule 6 and Alveolar Epithelial Cell Markers in Lungs of Human Infants with Chronic Lung Disease. J Histochem Cytochem 63:908-21
Raymond, Wilfred W; Xu, Xiang; Nimishakavi, Shilpa et al. (2015) Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux. PLoS One 10:e0125797
LaFemina, Michael J; Sutherland, Katherine M; Bentley, Trevor et al. (2014) Claudin-18 deficiency results in alveolar barrier dysfunction and impaired alveologenesis in mice. Am J Respir Cell Mol Biol 51:550-8
Gonzalez, Robert F; Dobbs, Leland G (2013) Isolation and culture of alveolar epithelial Type I and Type II cells from rat lungs. Methods Mol Biol 945:145-59
Chapin, Cheryl; Bailey, Nicole A; Gonzales, Linda W et al. (2012) Distribution and surfactant association of carcinoembryonic cell adhesion molecule 6 in human lung. Am J Physiol Lung Cell Mol Physiol 302:L216-25
Heine, Vivi M; Griveau, Amelie; Chapin, Cheryl et al. (2011) A small-molecule smoothened agonist prevents glucocorticoid-induced neonatal cerebellar injury. Sci Transl Med 3:105ra104
Gonzalez, Robert F; Allen, Lennell; Gonzales, Linda et al. (2010) HTII-280, a biomarker specific to the apical plasma membrane of human lung alveolar type II cells. J Histochem Cytochem 58:891-901

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