investigators, and her standing in the microscopy community at UCSF make her an exceptional asset to the Program Project. She will be responsible for the day-to-day operations of the Core Facility. FUNCTIONS OF THE MICROSCOPY AND IMAGE ANALYSIS CORE The Core Microscopy Laboratory allows investigators in the Program Project to carry out histological and cytological studies of optimal quality. The Core facility provides several different important functions that have been essential to the operation of the Program Project. These include the following. 1) It provides professional and technical staff capable of high quality, sophisticated microscopic, photographic, and computer graphic techniques. The staff collaborates with investigators from each of the projects in designing experiments and provides the technical expertise to perform the microscopic techniques and assist in the interpretation of the results. 2) The Core provides the equipment, darkroom, photographic supplies, image analysis and graphics software for doing a full range of morphological studies and ensures that these supplies are available and that the equipment is well maintained. This is a key function of the staff because much of the equipment is complex and expensive, may be easily damaged, or may render suboptimal

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL024075-31A1
Application #
8054537
Study Section
Special Emphasis Panel (ZHL1-PPG-S (05))
Project Start
2011-01-01
Project End
2015-12-31
Budget Start
2011-01-01
Budget End
2011-12-31
Support Year
31
Fiscal Year
2011
Total Cost
$233,429
Indirect Cost
Name
University of California San Francisco
Department
Type
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Barrette, Anne Marie; Roberts, Jessica K; Chapin, Cheryl et al. (2016) Antiinflammatory Effects of Budesonide in Human Fetal Lung. Am J Respir Cell Mol Biol 55:623-632
Danhaive, Olivier; Chapin, Cheryl; Horneman, Hart et al. (2015) Surface film formation in vitro by infant and therapeutic surfactants: role of surfactant protein B. Pediatr Res 77:340-6
Vanderbilt, Jeff N; Gonzalez, Robert F; Allen, Lennell et al. (2015) High-efficiency type II cell-enhanced green fluorescent protein expression facilitates cellular identification, tracking, and isolation. Am J Respir Cell Mol Biol 53:14-21
Gonzales, Linda W; Gonzalez, Robert; Barrette, Anne Marie et al. (2015) Expression of Carcinoembryonic Cell Adhesion Molecule 6 and Alveolar Epithelial Cell Markers in Lungs of Human Infants with Chronic Lung Disease. J Histochem Cytochem 63:908-21
Raymond, Wilfred W; Xu, Xiang; Nimishakavi, Shilpa et al. (2015) Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux. PLoS One 10:e0125797
LaFemina, Michael J; Sutherland, Katherine M; Bentley, Trevor et al. (2014) Claudin-18 deficiency results in alveolar barrier dysfunction and impaired alveologenesis in mice. Am J Respir Cell Mol Biol 51:550-8
Gonzalez, Robert F; Dobbs, Leland G (2013) Isolation and culture of alveolar epithelial Type I and Type II cells from rat lungs. Methods Mol Biol 945:145-59
Chapin, Cheryl; Bailey, Nicole A; Gonzales, Linda W et al. (2012) Distribution and surfactant association of carcinoembryonic cell adhesion molecule 6 in human lung. Am J Physiol Lung Cell Mol Physiol 302:L216-25
Heine, Vivi M; Griveau, Amelie; Chapin, Cheryl et al. (2011) A small-molecule smoothened agonist prevents glucocorticoid-induced neonatal cerebellar injury. Sci Transl Med 3:105ra104
Gonzalez, Robert F; Allen, Lennell; Gonzales, Linda et al. (2010) HTII-280, a biomarker specific to the apical plasma membrane of human lung alveolar type II cells. J Histochem Cytochem 58:891-901

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