The Lipid and Lipoprotein Biochemistry Core Laboratory will serve all projects on a highly interactive and collaborative basis. The primary role of this core unit is to provide phenotypic data on measures of plasma lipoproteins. This core unit also is responsible for processing and storing the blood samples used by the core unit and by Project 1. Quantitative measures of lipoprotein phenotypes will be made by clinical chemical and immunochemical techniques. Standard clinical chemical procedures will be used to quantify serum TC and HDL-C after precipitation. Immunoturbidimetric and other immunochemical techniques will be used to measure serum concentrations of apoAI, apoB, apoE, LP(A) particles bearing specific isoforms. In addition, we will determine apo(a) isoform phenotypes. The gradiant gel electrophoresis (GGE) technique, established in our laboratory for the specific purpose of genetic research involving large numbers of samples, will enable estimates of quantities of cholesterol in each of four size-resolved HDL particle classes: HDL1a, HDL1b HDL2, and HDL3. In addition, the GGE technique will be used to determine the distribution of cholesterol among four size-resolved LDL particle classes, as well as the median particle diameter and diameter of the predominant LDL species in each sample. Blood processing will involve isolating serum for phenotypic measures, processing it for storage as 60 mul aliquots, and storing clots as a backup source of DNA. Sera and clots will be catalogued in the computerized inventory database, maintained at -80 degrees Centigrade, and retrieved as needed. Blood from 750 patients and inbred progeny projected to undergo the dual dietary challenge will be processed and assayed for lipoprotein phenotypes on the occasions of the three dietary samplings. In addition, LP(a) phenotypes, apo(a) phenotypes, and LDL cholesterol distributions will be assayed on 1,800 samples from non-inbred animals that underwent the dual dietary challenge during the current project period.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Southwest Foundation for Biomedical Research
San Antonio
United States
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Mahaney, Michael C; Karere, Genesio M; Rainwater, David L et al. (2018) Diet-induced early-stage atherosclerosis in baboons: Lipoproteins, atherogenesis, and arterial compliance. J Med Primatol 47:3-17
Joganic, Jessica L; Willmore, Katherine E; Richtsmeier, Joan T et al. (2018) Additive genetic variation in the craniofacial skeleton of baboons (genus Papio) and its relationship to body and cranial size. Am J Phys Anthropol 165:269-285
Eichel, Kaleigh Anne; Ackermann, Rebecca Rogers (2016) Variation in the nasal cavity of baboon hybrids with implications for late Pleistocene hominins. J Hum Evol 94:134-45
Tiyasatkulkovit, Wacharaporn; Malaivijitnond, Suchinda; Charoenphandhu, Narattaphol et al. (2014) Pueraria mirifica extract and puerarin enhance proliferation and expression of alkaline phosphatase and type I collagen in primary baboon osteoblasts. Phytomedicine 21:1498-503
Chen, Shuyuan; Bastarrachea, Raul A; Roberts, Brad J et al. (2014) Successful ? cells islet regeneration in streptozotocin-induced diabetic baboons using ultrasound-targeted microbubble gene therapy with cyclinD2/CDK4/GLP1. Cell Cycle 13:1145-51
Higgins, Paul B; Rodriguez, Perla J; Voruganti, V Saroja et al. (2014) Body composition and cardiometabolic disease risk factors in captive baboons (Papio hamadryas sp.): sexual dimorphism. Am J Phys Anthropol 153:9-14
Rodríguez-Sánchez, I P; Garza-Rodríguez, M L; Mohamed-Noriega, K et al. (2013) Olfactomedin-like 3 (OLFML3) gene expression in baboon and human ocular tissues: cornea, lens, uvea, and retina. J Med Primatol 42:105-11
Fabbrini, Elisa; Higgins, Paul B; Magkos, Faidon et al. (2013) Metabolic response to high-carbohydrate and low-carbohydrate meals in a nonhuman primate model. Am J Physiol Endocrinol Metab 304:E444-51
Shi, Qiang; Hornsby, Peter J; Meng, Qinghe et al. (2013) Longitudinal analysis of short-term high-fat diet on endothelial senescence in baboons. Am J Cardiovasc Dis 3:107-19
Spradling, Kimberly D; Glenn, Jeremy P; Garcia, Roy et al. (2013) The baboon kidney transcriptome: analysis of transcript sequence, splice variants, and abundance. PLoS One 8:e57563

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