Abstract

The purpose of the Analytical and Morphological Core will be to centralize the following techniques: analytical chemistry, enzyme-linked immunoassays (ELISA), radioimmunoassays (RIA), Western blot analysis, and receptor binding assay. Analytical chemistry will be used to determine nitrites, nitrates and hydroxyproline. ELISA and RIA will be utilized for the measurement of plasma and tissue levels of the tetrapeptide Ac-SDKP, prostanoids, vasoactive peptide, cyclic adenosine and guanosine monophosphates. Wester blot analysis will be performed to measure metalloproteinase-2 (MMP-2) and MMP-9. Zymography will be used to determine metalloproteinase (MMP-2 and MMP-9) activities. EMSA will be utilized to assess activation of the nuclear transcription factor NF-kappa beta. For the morphometric analyses, Core Bi will prepare tissue slides (i.e., cryostat sectioning, embedding and histological and immunohistochemical staining) for the measurement of myocardial infarct size, cardiomyocyte size, capillary density, interstitial and perivascular collagen types 1 and 3, interstitial collagen volume fraction, apoptosis and necrosis. Finally, Quantitative in vitro autoradiography and fluorescnece/confocal microscopy will be utilized for radioreceptor binding assays and anatomical and cellular localization of membrane-bound, intracellular and nuclear receptors.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL028982-21
Application #
6649485
Study Section
Project Start
2002-09-01
Project End
2007-08-31
Budget Start
Budget End
Support Year
21
Fiscal Year
2002
Total Cost
$73,467
Indirect Cost

  Institution

Name
Henry Ford Health System
Department
Type
DUNS #
073134603
City
Detroit
State
MI
Country
United States
Zip Code
48202

  Publications

Kumar, Nitin; Liao, Tang-Dong; Romero, Cesar A et al. (2018) Thymosin ?4 Deficiency Exacerbates Renal and Cardiac Injury in Angiotensin-II-Induced Hypertension. Hypertension 71:1133-1142
Bryson, Timothy D; Gu, Xiaosong; Khalil, Remonda M et al. (2018) Overexpression of prostaglandin E2 EP4 receptor improves cardiac function after myocardial infarction. J Mol Cell Cardiol 118:1-12
Cerniello, Flavia M; Carretero, Oscar A; Longo Carbajosa, Nadia A et al. (2017) MAS1 Receptor Trafficking Involves ERK1/2 Activation Through a ?-Arrestin2-Dependent Pathway. Hypertension 70:982-989
Ramseyer, Vanesa D; Ortiz, Pablo A; Carretero, Oscar A et al. (2016) Angiotensin II-mediated hypertension impairs nitric oxide-induced NKCC2 inhibition in thick ascending limbs. Am J Physiol Renal Physiol 310:F748-F754
González, Germán E; Rhaleb, N-E; D'Ambrosio, Martin A et al. (2016) Cardiac-deleterious role of galectin-3 in chronic angiotensin II-induced hypertension. Am J Physiol Heart Circ Physiol 311:H1287-H1296
Gu, Xiaosong; Xu, Jiang; Zhu, Liping et al. (2016) Prostaglandin E2 Reduces Cardiac Contractility via EP3 Receptor. Circ Heart Fail 9:
Kumar, Nitin; Nakagawa, Pablo; Janic, Branislava et al. (2016) The anti-inflammatory peptide Ac-SDKP is released from thymosin-?4 by renal meprin-? and prolyl oligopeptidase. Am J Physiol Renal Physiol 310:F1026-34
Zhu, Liping; Yang, Xiao-Ping; Janic, Branislava et al. (2016) Ac-SDKP suppresses TNF-?-induced ICAM-1 expression in endothelial cells via inhibition of I?B kinase and NF-?B activation. Am J Physiol Heart Circ Physiol 310:H1176-83
Saez, Fara; Hong, Nancy J; Garvin, Jeffrey L (2016) Luminal flow induces NADPH oxidase 4 translocation to the nuclei of thick ascending limbs. Physiol Rep 4:
Cerrato, Bruno D; Carretero, Oscar A; Janic, Brana et al. (2016) Heteromerization Between the Bradykinin B2 Receptor and the Angiotensin-(1-7) Mas Receptor: Functional Consequences. Hypertension 68:1039-48

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