About 30% of the US population is hypertensive, and a large portion is salt-sensitive. Activation of angiotensin II (Ang II) type 1 receptors (ATi) promotes salt retention by stimulating nephron transport and constricting renal vessels, while stimulating type 2 receptors (AT2) promotes salt excretion. ImlDalances favoring the former increase blood pressure (BP), and end organ (damage (EOD). Thick ascending limbs (THALs) reabsorb 25-30% of filtered NaCl through both trans- and paracellular pathways. We and others reported that ATi activation stimulated protein kinase C and O2"""""""", both of which usually enhance THAL NaCl absorption. We also showed that stimulating AT2 enhanced NO production. NO inhibits NaCl absorption by: 1) activating phosphodiesterase II (PDE II), which degrades cAMP;and 2) by reducing 02'. However, the role of ATi in stimulating THAL NaCl absorption is not well understood, and whether AT2 activation inhibits transport is unknown. Elevated THAL NaCl absorption and defects in THAL NO production and signaling contribute to salt-sensitive hypertension and renal damage, but we do not know whether AT2-induced NO signaling is defective as well. We hypothesize that in animals on a normal diet the stimulatory effects ofATi activation on THAL NaCl absorption are blunted by AT2-induced NO which reduces cAMP and O2'. Defects in THAL AT2 signaling promote increases in BP, salt-sensitive hypertension and renal damage.
Aim I tests whether the levels of Ang II found in vivo in the renal outer medulla activate AT2 which blunt the stimulatory effects of ATi activation on net NaCl absorption by inhibiting trans- and paracellular transport.
Aim II tests whether AT2 activation augments NO and cGMP, and cGMP reduces NaCl absorption in part by enhancing PDE II activity which reduces cAMP.
Aim III tests whether AT2-induced NO and cGMP reduces NaCl absorption in part by diminishing ATi-stimulated O2"""""""".
Aim I V tests whether AT2-induced inhibition of THAL NaCl transport is reduced in salt-sensitive hypertension and the mechanisms involved.
These aims will be addressed using cellular and molecular biological techniques in addition to whole animal experiments. This project relates to Project I because both will study Dahl SS rats. It relates to Project II because both will study Ang II and O2"""""""". It relates to Projects III because both will study: 1) Na transport;2) Ang II;and 3) Dahl rats. Project IV will use the Administrative, Mutant Mouse, Analytical and Biostatistical Cores.
This project is important because it addresses how Ang II regulates THAL NaCl absorption through activation of ATi and AT2 receptors. Defects in THAL NaCl absorption cause hypertension, especially salt- sensitive hypertension, and renal damage. Understanding the cellular mechanisms by which AT2 activation reduces THAL transport and how salt-sensitive hypertension changes them may lead to better treatments for this disease and for the renal damage caused by high BP.
|Kumar, Nitin; Liao, Tang-Dong; Romero, Cesar A et al. (2018) Thymosin ?4 Deficiency Exacerbates Renal and Cardiac Injury in Angiotensin-II-Induced Hypertension. Hypertension 71:1133-1142|
|Bryson, Timothy D; Gu, Xiaosong; Khalil, Remonda M et al. (2018) Overexpression of prostaglandin E2 EP4 receptor improves cardiac function after myocardial infarction. J Mol Cell Cardiol 118:1-12|
|Cerniello, Flavia M; Carretero, Oscar A; Longo Carbajosa, Nadia A et al. (2017) MAS1 Receptor Trafficking Involves ERK1/2 Activation Through a ?-Arrestin2-Dependent Pathway. Hypertension 70:982-989|
|Ramseyer, Vanesa D; Ortiz, Pablo A; Carretero, Oscar A et al. (2016) Angiotensin II-mediated hypertension impairs nitric oxide-induced NKCC2 inhibition in thick ascending limbs. Am J Physiol Renal Physiol 310:F748-F754|
|González, Germán E; Rhaleb, N-E; D'Ambrosio, Martin A et al. (2016) Cardiac-deleterious role of galectin-3 in chronic angiotensin II-induced hypertension. Am J Physiol Heart Circ Physiol 311:H1287-H1296|
|Gu, Xiaosong; Xu, Jiang; Zhu, Liping et al. (2016) Prostaglandin E2 Reduces Cardiac Contractility via EP3 Receptor. Circ Heart Fail 9:|
|Kumar, Nitin; Nakagawa, Pablo; Janic, Branislava et al. (2016) The anti-inflammatory peptide Ac-SDKP is released from thymosin-?4 by renal meprin-? and prolyl oligopeptidase. Am J Physiol Renal Physiol 310:F1026-34|
|Zhu, Liping; Yang, Xiao-Ping; Janic, Branislava et al. (2016) Ac-SDKP suppresses TNF-?-induced ICAM-1 expression in endothelial cells via inhibition of I?B kinase and NF-?B activation. Am J Physiol Heart Circ Physiol 310:H1176-83|
|Saez, Fara; Hong, Nancy J; Garvin, Jeffrey L (2016) Luminal flow induces NADPH oxidase 4 translocation to the nuclei of thick ascending limbs. Physiol Rep 4:|
|Cerrato, Bruno D; Carretero, Oscar A; Janic, Brana et al. (2016) Heteromerization Between the Bradykinin B2 Receptor and the Angiotensin-(1-7) Mas Receptor: Functional Consequences. Hypertension 68:1039-48|
Showing the most recent 10 out of 381 publications