Abstract

Core B is a blood cell separation and MHC typing Core. The facility comprises two parts: each has devoted hoods, incubators and liquid nitrogen and -80 degrees C freezers. The first part performs all sample processing from whole blood to PBMC, plasma and serum samples and the freezing of these samples. When required for individual requirements of the Projects, the first part of Core B also separates B, T, NK, NKT, of Core B carries out MHC allele typing, including complement typing, and EBV transformation of B cells to generate, bank and maintain B lymphoblastoid cell lines. The basic typing regimen includes DNA typing by PCR and hybridization with oligonucleotide probes of HLA-A, B, C, DRB (including high resolution as needed), DQA, DQB, DPA and DPB and protein typing for BF, C2, C4A and C4B. Part 2 of Core B also performs ABO blood group typing as needed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL029583-20
Application #
6993644
Study Section
Project Start
2004-12-01
Project End
2005-11-30
Budget Start
2004-12-01
Budget End
2005-11-30
Support Year
20
Fiscal Year
2005
Total Cost
$233,236
Indirect Cost

  Institution

Name
Immune Disease Institute, Inc.
Department
Type
DUNS #
059709394
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Feris, Edmond J; Encinales, Liliana; Awad, Carlos et al. (2016) High levels of anti-tuberculin (IgG) antibodies correlate with the blocking of T-cell proliferation in individuals with high exposure to Mycobacterium tuberculosis. Int J Infect Dis 43:21-24
Larsen, Charles E; Alford, Dennis R; Trautwein, Michael R et al. (2014) Dominant sequences of human major histocompatibility complex conserved extended haplotypes from HLA-DQA2 to DAXX. PLoS Genet 10:e1004637
Granados-Montiel, Julio; Zuniga, Joaquin; Azocar, Jose et al. (2011) Interaction between immunoglobulin allotypes and NK receptor genes in diabetes post-hepatitis C virus infection. Immunobiology 216:686-91
Encinales, Liliana; Zuniga, Joaquin; Granados-Montiel, Julio et al. (2010) Humoral immunity in tuberculin skin test anergy and its role in high-risk persons exposed to active tuberculosis. Mol Immunol 47:1066-73
Szilagyi, Agnes; Banlaki, Zsofia; Pozsonyi, Eva et al. (2010) Frequent occurrence of conserved extended haplotypes (CEHs) in two Caucasian populations. Mol Immunol 47:1899-904
Zúñiga, Joaquín; Romero, Viviana; Azocar, José et al. (2009) Protective KIR-HLA interactions for HCV infection in intravenous drug users. Mol Immunol 46:2723-7
Stern, Joel N H; Keskin, Derin B; Romero, Viviana et al. (2009) Molecular signatures distinguishing active from latent tuberculosis in peripheral blood mononuclear cells, after in vitro antigenic stimulation with purified protein derivative of tuberculin (PPD) or Candida: a preliminary report. Immunol Res 45:1-12
Husain, Zaheed; Kelly, M Ann; Eisenbarth, George S et al. (2008) The MHC type 1 diabetes susceptibility gene is centromeric to HLA-DQB1. J Autoimmun 30:266-72
Romero, Viviana; Zuniga, Joaquin; Azocar, Jose et al. (2008) Genetic interactions of KIR and G1M immunoglobulin allotypes differ in obese from non-obese individuals with type 2 diabetes. Mol Immunol 45:3857-62
Romero, Viviana; Azocar, Jose; Zuniga, Joaquin et al. (2008) Interaction of NK inhibitory receptor genes with HLA-C and MHC class II alleles in Hepatitis C virus infection outcome. Mol Immunol 45:2429-36

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