Abstract

Core C is a continuing core for Trangenic and Gene Targeting of Mice. In the next grant cycle Core C will assist Project 1 in constructing endothelial specific knockouts of SCAP and STATS on the LDL receptor null background.Core C will assist Projects 1 arid 2 in constructing HO-1 conditional and endothelial specific, knockout of HO-1 on the apoE null background. Core C will assist Project 3 in construction of a MGP-P64Q mutant mouse through gene targeting and will assist Project 3 in the generation of Abcc6-Wnt-promoterbeta- gal reporter double transgenic mice. Core C will provide consultation on breeding and colony management of transgenic mouse models to Project 4. Core C will assist Project 5 construction of, transgenci mice for testing atherosclerosis-related candidate genes. Core C will assist Project 6 with the construction of transgenic mice expressing dominant negatie NR4A receptor in macrophages. Core C will , also provide assistance to Core A in the characterization of primary cultues of mouse aortic endothelial cells by performing qPCR and Western blot analysis.These functions of Core C are essential to ensuring the efficient and cost-effective completion of the specific aims of all of the component Projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL030568-27
Application #
8048093
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2010-04-01
Budget End
2011-03-31
Support Year
27
Fiscal Year
2010
Total Cost
$198,002
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Kang, Eun Yong; Lee, Cue Hyunkyu; Furlotte, Nicholas A et al. (2018) An Association Mapping Framework To Account for Potential Sex Difference in Genetic Architectures. Genetics 209:685-698
Seldin, Marcus M; Koplev, Simon; Rajbhandari, Prashant et al. (2018) A Strategy for Discovery of Endocrine Interactions with Application to Whole-Body Metabolism. Cell Metab 27:1138-1155.e6
Wang, Bo; Rong, Xin; Palladino, Elisa N D et al. (2018) Phospholipid Remodeling and Cholesterol Availability Regulate Intestinal Stemness and Tumorigenesis. Cell Stem Cell 22:206-220.e4
Kasahara, Kazuyuki; Krautkramer, Kimberly A; Org, Elin et al. (2018) Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model. Nat Microbiol 3:1461-1471
Lang, Jennifer M; Pan, Calvin; Cantor, Rita M et al. (2018) Impact of Individual Traits, Saturated Fat, and Protein Source on the Gut Microbiome. MBio 9:
McDonald, Austin I; Shirali, Aditya S; Aragón, Raquel et al. (2018) Endothelial Regeneration of Large Vessels Is a Biphasic Process Driven by Local Cells with Distinct Proliferative Capacities. Cell Stem Cell 23:210-225.e6
Roberts, Adam B; Gu, Xiaodong; Buffa, Jennifer A et al. (2018) Development of a gut microbe-targeted nonlethal therapeutic to inhibit thrombosis potential. Nat Med 24:1407-1417
Zhu, W; Buffa, J A; Wang, Z et al. (2018) Flavin monooxygenase 3, the host hepatic enzyme in the metaorganismal trimethylamine N-oxide-generating pathway, modulates platelet responsiveness and thrombosis risk. J Thromb Haemost 16:1857-1872
Olde Loohuis, Loes M; Mangul, Serghei; Ori, Anil P S et al. (2018) Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia. Transl Psychiatry 8:96
Mukherjee, Pallavi; Hough, Greg; Chattopadhyay, Arnab et al. (2018) Role of enterocyte stearoyl-Co-A desaturase-1 in LDLR-null mice. J Lipid Res 59:1818-1840

Showing the most recent 10 out of 791 publications