Abstract

Core B will provide services to the component projects in three areas of expertise. (1) Analytical Chemistry related services: Core B will perform quantitative analysis of lipids, lipoproteins, hormones, enzyme activities, and various plasma metabolites that have relevance to atherosclerosis and lipid metabolism. (2) Molecular Biology related services: Core B will assist in the construction of novel, genetically modified animal models by gene targeting and transgenic mouse techniques. This core will also help in the construction of recombinant adenoviruses. (3) Morphology related services: Core B will provide quantitative and detailed cellular analysis of atherosclerotic lesions in the aortic root and innominate arteries of mice and will provide quantitative analysis of the percent of aorta with atherosclerotic lesions using en face analysis. Core B will also offer immunocytochemistry and immunohistology related services. All component projects are expected to use the services provided by Core B in the next grant cycle.

Public Health Relevance

The core assists the component projects in generating genetically modified animal models and performing various tests on samples collected from animals, tissues, and cells for the study of lipid and lipoprotein metabolism in atherosclerosis. This is highly relevant to public health since atherosclerosis is a major cause for cardiovascular diseases such as coronary heart disease and stroke. Findings from this program project can potentially lead to the development of novel therapeutics for cardiovascular disease.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL030568-35
Application #
9689090
Study Section
Special Emphasis Panel (ZHL1)
Program Officer
Liu, Lijuan
Project Start
Project End
Budget Start
2019-05-01
Budget End
2020-04-30
Support Year
35
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

McDonald, Austin I; Shirali, Aditya S; Aragón, Raquel et al. (2018) Endothelial Regeneration of Large Vessels Is a Biphasic Process Driven by Local Cells with Distinct Proliferative Capacities. Cell Stem Cell 23:210-225.e6
Roberts, Adam B; Gu, Xiaodong; Buffa, Jennifer A et al. (2018) Development of a gut microbe-targeted nonlethal therapeutic to inhibit thrombosis potential. Nat Med 24:1407-1417
Zhu, W; Buffa, J A; Wang, Z et al. (2018) Flavin monooxygenase 3, the host hepatic enzyme in the metaorganismal trimethylamine N-oxide-generating pathway, modulates platelet responsiveness and thrombosis risk. J Thromb Haemost 16:1857-1872
Olde Loohuis, Loes M; Mangul, Serghei; Ori, Anil P S et al. (2018) Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia. Transl Psychiatry 8:96
Mukherjee, Pallavi; Hough, Greg; Chattopadhyay, Arnab et al. (2018) Role of enterocyte stearoyl-Co-A desaturase-1 in LDLR-null mice. J Lipid Res 59:1818-1840
Orozco, Luz D; Farrell, Colin; Hale, Christopher et al. (2018) Epigenome-wide association in adipose tissue from the METSIM cohort. Hum Mol Genet 27:1830-1846
Erbilgin, Ayca; Seldin, Marcus M; Wu, Xiuju et al. (2018) Transcription Factor Zhx2 Deficiency Reduces Atherosclerosis and Promotes Macrophage Apoptosis in Mice. Arterioscler Thromb Vasc Biol 38:2016-2027
Boström, Kristina I; Yao, Jiayi; Wu, Xiuju et al. (2018) Endothelial Cells May Have Tissue-Specific Origins. J Cell Biol Histol 1:
Norheim, Frode; Bjellaas, Thomas; Hui, Simon T et al. (2018) Genetic, dietary, and sex-specific regulation of hepatic ceramides and the relationship between hepatic ceramides and IR. J Lipid Res 59:1164-1174
Yu, Jingyi; Seldin, Marcus M; Fu, Kai et al. (2018) Topological Arrangement of Cardiac Fibroblasts Regulates Cellular Plasticity. Circ Res 123:73-85

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