The Morphology, Immunology and Molecular Biology Core will provide the various projects in the program with the necessary expertise, facilities, and support to perform routing light microscopy, immunofluorescent, microscopy, and other routine and specialized morphological, pathological and immunohistochemical support required The Molecular Biology component of the Core will include the growth and purification of DNA, proteins and recombinant viruses;site directed mutagenesis of cDNA, production of transfection vectors and plasmids, expression and analysis of channel proteins in mammalian cell cultures. The core will also be the primary facility for the production and maintenance of the transgenic mouse colonies. These responsibilities will include breeding, genotyping and phenotypic analysis by immunochemical means.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL039707-20
Application #
8122103
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2010-09-01
Budget End
2011-08-31
Support Year
20
Fiscal Year
2010
Total Cost
$351,773
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
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Herron, Todd J; Rocha, Andre Monteiro Da; Campbell, Katherine F et al. (2016) Extracellular Matrix-Mediated Maturation of Human Pluripotent Stem Cell-Derived Cardiac Monolayer Structure and Electrophysiological Function. Circ Arrhythm Electrophysiol 9:e003638
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Willis, B Cicero; Pandit, Sandeep V; Ponce-Balbuena, Daniela et al. (2016) Constitutive Intracellular Na+ Excess in Purkinje Cells Promotes Arrhythmogenesis at Lower Levels of Stress Than Ventricular Myocytes From Mice With Catecholaminergic Polymorphic Ventricular Tachycardia. Circulation 133:2348-59

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