The Analytical Instrumentation Core will provide all the projects and investigators of the Program Project with a variety of state-of-the-art instrumentation aimed at obtaining measurements in various cells of cell metabolism, ion fluxes, compartmentation of ionic gradients and metabolites, intracellular messengers, etc. as described in the individual sections of the proposal. The instrumentation available through Core C is comprised of several spectrophotometers, fluorometers, HPLC systems, electrometers, amperometric detectors, etc. Part of the instrumentation has been produced commercially and part has been built inhouse and is continually upgraded to fulfill specific research needs. the staff of the core, who has proven expertise in the measurements proposed and in developing state-of-the-art technology, will provide assistance and counseling in the measurements described in the individual projects of this application. The measurements that Core C will carry out include: a) measurements of free intracellular H+ and Ca2+ in cell suspensions, cell monolayers and individual cells; b) kinetic measurement of ion transport across cell and cell membranes; c) microfluorimetric assays of P450 and other enzymatic reaction in small volumes or few cells; d) imaging of cell fluorescence (single cells or several cells) for H+or Ca2+ free intracellular distribution, fluorescent antibody distribution, etc.; e) ion selective electrodes, atomic absorbance, cell permeabilization, etc.; and f) measurements of cell metabolism; ATP/ADP ratios; HPLC of phosphomeabolites, phosphoinositides, etc.
| Huang, Chunfa; Miller, Richard Tyler (2010) Novel Ca receptor signaling pathways for control of renal ion transport. Curr Opin Nephrol Hypertens 19:106-12 |
| Yu, Changqing; Yang, Zhiwei; Ren, Hongmei et al. (2009) D3 dopamine receptor regulation of ETB receptors in renal proximal tubule cells from WKY and SHRs. Am J Hypertens 22:877-83 |
| Zeng, Chunyu; Asico, Laureano D; Yu, Changqing et al. (2008) Renal D3 dopamine receptor stimulation induces natriuresis by endothelin B receptor interactions. Kidney Int 74:750-9 |
| Zeng, Chunyu; Armando, Ines; Luo, Yingjin et al. (2008) Dysregulation of dopamine-dependent mechanisms as a determinant of hypertension: studies in dopamine receptor knockout mice. Am J Physiol Heart Circ Physiol 294:H551-69 |
| Zeng, Chunyu; Villar, Van Anthony M; Eisner, Gilbert M et al. (2008) G protein-coupled receptor kinase 4: role in blood pressure regulation. Hypertension 51:1449-55 |
| Resnick, Andrew; Hopfer, Ulrich (2008) Mechanical stimulation of primary cilia. Front Biosci 13:1665-80 |
| Woost, Philip G; Kolb, Robert J; Chang, Chung-Ho et al. (2007) Development of an AT2-deficient proximal tubule cell line for transport studies. In Vitro Cell Dev Biol Anim 43:352-60 |
| Resnick, Andrew; Hopfer, Ulrich (2007) Force-response considerations in ciliary mechanosensation. Biophys J 93:1380-90 |
| Huang, Chunfa; Miller, R Tyler (2007) Regulation of renal ion transport by the calcium-sensing receptor: an update. Curr Opin Nephrol Hypertens 16:437-43 |
| Ulmasov, Barbara; Bruno, Jonathan; Woost, Philip G et al. (2007) Tissue and subcellular distribution of CLIC1. BMC Cell Biol 8:8 |
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