Abstract

The objective of this Core resources is to provide routine molecular biological services to Program investigators. Clones of rat and human cardiac K+ and Na+ channels have been sequenced from ventricular muscle and from genomic libraries. These have been expressed in Xenopus oocytes and in a stable mammalian cell line, mouse Ltk cells. This Core will make cDNA expression vectors or mRNA for native or mutated channels for expression studies and will be responsible for transfecting L-cells with native or mutated channel cDNA. Growing and maintaining cultures of successfully transfected cell lines will be undertaken by the Cellular Resources facility (Core A). Thus, Core B will centralize routine and cost-intensive molecular biologic tasks, and therefore resources and investigator effort will be used most effectively.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
1P01HL046681-01A1
Application #
3844971
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212

  Publications

Yang, Tao; Smith, Jarrod A; Leake, Brenda F et al. (2013) An allosteric mechanism for drug block of the human cardiac potassium channel KCNQ1. Mol Pharmacol 83:481-9
Hayashi, Kenshi; Shuai, Wen; Sakamoto, Yuichiro et al. (2010) Trafficking-competent KCNQ1 variably influences the function of HERG long QT alleles. Heart Rhythm 7:973-80
Yang, Tao; McBride, Brian F; Leake, Brenda F et al. (2010) Modulation of drug block of the cardiac potassium channel KCNA5 by the drug transporters OCTN1 and MDR1. Br J Pharmacol 161:1023-33
Stepanovic, Svetlana Z; Potet, Franck; Petersen, Christina I et al. (2009) The evolutionarily conserved residue A653 plays a key role in HERG channel closing. J Physiol 587:2555-66
Yang, Tao; Chung, Seo-Kyung; Zhang, Wei et al. (2009) Biophysical properties of 9 KCNQ1 mutations associated with long-QT syndrome. Circ Arrhythm Electrophysiol 2:417-26
Potet, Franck; Petersen, Christina I; Boutaud, Olivier et al. (2009) Genetic screening in C. elegans identifies rho-GTPase activating protein 6 as novel HERG regulator. J Mol Cell Cardiol 46:257-67
Yang, Tao; Kanki, Hideaki; Zhang, Wei et al. (2009) Probing the mechanisms underlying modulation of quinidine sensitivity to cardiac I(Ks) block by protein kinase A-mediated I(Ks) phosphorylation. Br J Pharmacol 157:952-61
Grueter, Chad E; Abiria, Sunday A; Wu, Yunji et al. (2008) Differential regulated interactions of calcium/calmodulin-dependent protein kinase II with isoforms of voltage-gated calcium channel beta subunits. Biochemistry 47:1760-7
Baudenbacher, Franz; Schober, Tilmann; Pinto, Jose Renato et al. (2008) Myofilament Ca2+ sensitization causes susceptibility to cardiac arrhythmia in mice. J Clin Invest 118:3893-903
Makita, Naomasa; Behr, Elijah; Shimizu, Wataru et al. (2008) The E1784K mutation in SCN5A is associated with mixed clinical phenotype of type 3 long QT syndrome. J Clin Invest 118:2219-29

Showing the most recent 10 out of 171 publications