Abstract

This Core will perform cell isolations and culture procedures for investigators in Projects 2, 3, and 4. The Core will be responsible for isolating and maintaining cardiac myocytes, isolating and maintaining Xenopus laevis oocytes, and carrying out either cytoplasmic injections of mRNA or nuclear injections of DNA provided by the Molecular Biology Core (Core B). Projects 2 and 3 both propose studies of human and rat potassium channels expressed in mouse Ltk cells; this Core will assume the responsibility of growing and maintaining these cultures after the initial transfections have been performed in Core B. By centralizing these routine laboratory functions, resources (investigator effort, animal numbers and costs) will be used more effectively.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL046681-04
Application #
3736934
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212

  Publications

Yang, Tao; Smith, Jarrod A; Leake, Brenda F et al. (2013) An allosteric mechanism for drug block of the human cardiac potassium channel KCNQ1. Mol Pharmacol 83:481-9
Hayashi, Kenshi; Shuai, Wen; Sakamoto, Yuichiro et al. (2010) Trafficking-competent KCNQ1 variably influences the function of HERG long QT alleles. Heart Rhythm 7:973-80
Yang, Tao; McBride, Brian F; Leake, Brenda F et al. (2010) Modulation of drug block of the cardiac potassium channel KCNA5 by the drug transporters OCTN1 and MDR1. Br J Pharmacol 161:1023-33
Stepanovic, Svetlana Z; Potet, Franck; Petersen, Christina I et al. (2009) The evolutionarily conserved residue A653 plays a key role in HERG channel closing. J Physiol 587:2555-66
Yang, Tao; Chung, Seo-Kyung; Zhang, Wei et al. (2009) Biophysical properties of 9 KCNQ1 mutations associated with long-QT syndrome. Circ Arrhythm Electrophysiol 2:417-26
Potet, Franck; Petersen, Christina I; Boutaud, Olivier et al. (2009) Genetic screening in C. elegans identifies rho-GTPase activating protein 6 as novel HERG regulator. J Mol Cell Cardiol 46:257-67
Yang, Tao; Kanki, Hideaki; Zhang, Wei et al. (2009) Probing the mechanisms underlying modulation of quinidine sensitivity to cardiac I(Ks) block by protein kinase A-mediated I(Ks) phosphorylation. Br J Pharmacol 157:952-61
Makita, Naomasa; Behr, Elijah; Shimizu, Wataru et al. (2008) The E1784K mutation in SCN5A is associated with mixed clinical phenotype of type 3 long QT syndrome. J Clin Invest 118:2219-29
Werdich, Andreas A; Lima, Eduardo A; Dzhura, Igor et al. (2008) Differential effects of phospholamban and Ca2+/calmodulin-dependent kinase II on [Ca2+]i transients in cardiac myocytes at physiological stimulation frequencies. Am J Physiol Heart Circ Physiol 294:H2352-62
Yang, P; Koopmann, T T; Pfeufer, A et al. (2008) Polymorphisms in the cardiac sodium channel promoter displaying variant in vitro expression activity. Eur J Hum Genet 16:350-7

Showing the most recent 10 out of 171 publications