Abstract

The Scientific Core will provide oligonucleotide synthesis and DNA sequencing. An oligonucleotide synthesizer is already in place, and a DNA sequencer will be purchased. All Projects in the Program will make extensive use of both procedures for applications such as the site-directed mutagenesis, cloning and genetic mutation analysis. Centralizing these capabilities in a Core facility offers not only economies of scale, but also rapid turnaround to allow the experiments to go forward in a timely fashion.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL046681-09
Application #
6355579
Study Section
Project Start
2000-08-01
Project End
2001-07-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
9
Fiscal Year
2000
Total Cost
$212,504
Indirect Cost

  Institution

Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212

  Publications

Yang, Tao; Smith, Jarrod A; Leake, Brenda F et al. (2013) An allosteric mechanism for drug block of the human cardiac potassium channel KCNQ1. Mol Pharmacol 83:481-9
Hayashi, Kenshi; Shuai, Wen; Sakamoto, Yuichiro et al. (2010) Trafficking-competent KCNQ1 variably influences the function of HERG long QT alleles. Heart Rhythm 7:973-80
Yang, Tao; McBride, Brian F; Leake, Brenda F et al. (2010) Modulation of drug block of the cardiac potassium channel KCNA5 by the drug transporters OCTN1 and MDR1. Br J Pharmacol 161:1023-33
Stepanovic, Svetlana Z; Potet, Franck; Petersen, Christina I et al. (2009) The evolutionarily conserved residue A653 plays a key role in HERG channel closing. J Physiol 587:2555-66
Yang, Tao; Chung, Seo-Kyung; Zhang, Wei et al. (2009) Biophysical properties of 9 KCNQ1 mutations associated with long-QT syndrome. Circ Arrhythm Electrophysiol 2:417-26
Potet, Franck; Petersen, Christina I; Boutaud, Olivier et al. (2009) Genetic screening in C. elegans identifies rho-GTPase activating protein 6 as novel HERG regulator. J Mol Cell Cardiol 46:257-67
Yang, Tao; Kanki, Hideaki; Zhang, Wei et al. (2009) Probing the mechanisms underlying modulation of quinidine sensitivity to cardiac I(Ks) block by protein kinase A-mediated I(Ks) phosphorylation. Br J Pharmacol 157:952-61
Makita, Naomasa; Behr, Elijah; Shimizu, Wataru et al. (2008) The E1784K mutation in SCN5A is associated with mixed clinical phenotype of type 3 long QT syndrome. J Clin Invest 118:2219-29
Werdich, Andreas A; Lima, Eduardo A; Dzhura, Igor et al. (2008) Differential effects of phospholamban and Ca2+/calmodulin-dependent kinase II on [Ca2+]i transients in cardiac myocytes at physiological stimulation frequencies. Am J Physiol Heart Circ Physiol 294:H2352-62
Yang, P; Koopmann, T T; Pfeufer, A et al. (2008) Polymorphisms in the cardiac sodium channel promoter displaying variant in vitro expression activity. Eur J Hum Genet 16:350-7

Showing the most recent 10 out of 171 publications