Experiments proposed in this Program Project application will heavily use genetically altered animalsalready produced in our laboratories, or obtained from other investigators, or from commercial laboratories.More than 15 mouse lines, including transgenic, knock-in and knock-out mice, are proposed in the overallproject.The mouse genetics core will maintain the minimum adequate numbers of animals for breeding for eachine, set timed pregnancy breeding as requested by the PI in each project, weaning, pup ID (ear punching,tagging), collection of tissue for genotyping, genomic DNA purification, establishing PCR conditions forgenotyping, performing PCR analysis, generating homozygous lines or transgenic lines carrying two or moretransgenes by adequate crossing, record keeping, link between the LASC and the Investigators in theProgram Project.The intent of the mouse genetics core is to efficiently generate sufficient numbers of experimental miceaccurately genotyped for all projects. By centralizing these activities this core will eliminate duplication ofeffort and animals by creating a central archive, storage and distribution system for all animals studied.Also it will make efficient use of equipment, will provide easy access to animal information, standardizedbreeding techniques and timing of set pregnancies as the investigators and technician assigned to the corehave extensive experience in genetically modified mouse and colony management.
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