The Molecular Biology Core will be used by subprojects 1, 2, 4, and 6. The core will provide services that will greatly facilitate the research proposed by subprojects 1 and 6, and more importantly, the core will allow subprojects 2 and 4 access to methodologies absent from there own laboratories. Access to such a core is essential because implementation of these technologies in each investigators' laboratory would be prohibitively expensive. For example, Dr. Coronado (subproject 2) wishes to obtain a reliable and abundant supply of certain toxins, from the Mexican breaded lizard Heloderma horridum horridum, that will be used as tools to dissect the function of the ryanodine receptor in cardiac muscle. Services provided by the Molecular Biology Core will include isolating the toxin cDNAs, determining the DNA sequence and constructing expression vectors that will permit the production of the toxins in bacteria. The expression and purification of these recombinant toxins will be done by the Protein Biochemistry Core (Core B). Dr. Coronado does not have either the equipment or the expertise to allow this to be done in his own laboratory. However, the use of the two core services will enable this project to be completed in a timely manner.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL047053-04
Application #
6272916
Study Section
Project Start
1998-01-01
Project End
1998-12-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
4
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Druckenbrod, Noah R; Powers, Patricia A; Bartley, Christopher R et al. (2008) Targeting of endothelin receptor-B to the neural crest. Genesis 46:396-400
Brickson, S; Fitzsimons, D P; Pereira, L et al. (2007) In vivo left ventricular functional capacity is compromised in cMyBP-C null mice. Am J Physiol Heart Circ Physiol 292:H1747-54
Balijepalli, Ravi C; Foell, Jason D; Hall, Duane D et al. (2006) Localization of cardiac L-type Ca(2+) channels to a caveolar macromolecular signaling complex is required for beta(2)-adrenergic regulation. Proc Natl Acad Sci U S A 103:7500-5
Stelzer, Julian E; Dunning, Sandy B; Moss, Richard L (2006) Ablation of cardiac myosin-binding protein-C accelerates stretch activation in murine skinned myocardium. Circ Res 98:1212-8
Martinez-Fernandez, Sergio; Hernandez-Torres, Francisco; Franco, Diego et al. (2006) Pitx2c overexpression promotes cell proliferation and arrests differentiation in myoblasts. Dev Dyn 235:2930-9
Stelzer, Julian E; Moss, Richard L (2006) Contributions of stretch activation to length-dependent contraction in murine myocardium. J Gen Physiol 128:461-71
Vatta, Matteo; Ackerman, Michael J; Ye, Bin et al. (2006) Mutant caveolin-3 induces persistent late sodium current and is associated with long-QT syndrome. Circulation 114:2104-12
Stelzer, Julian E; Larsson, Lars; Fitzsimons, Daniel P et al. (2006) Activation dependence of stretch activation in mouse skinned myocardium: implications for ventricular function. J Gen Physiol 127:95-107
Stelzer, Julian E; Fitzsimons, Daniel P; Moss, Richard L (2006) Ablation of myosin-binding protein-C accelerates force development in mouse myocardium. Biophys J 90:4119-27
Singla, Dinender K; Hacker, Timothy A; Ma, Lining et al. (2006) Transplantation of embryonic stem cells into the infarcted mouse heart: formation of multiple cell types. J Mol Cell Cardiol 40:195-200

Showing the most recent 10 out of 103 publications