Abstract

The development and application in vivo of artificial oxygen carriers of known and controllable characteristics offers a new approach to the exploration of uptake, delivery and utilization of oxygen by the various tissues. We will focus our efforts on the production of artificial hemoglobin-based oxygen carriers with highly controlled physico-chemical and functional properties, and test their effects on specific animal models. It is a highly interdisciplinary effort which involves molecular simulations, crystallography, genetic engineering, chemical synthesis, renal function tests and brain physiology. The strategy of the program includes two main components: Molecular design and in vivo applications.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL048517-02
Application #
2224573
Study Section
Heart, Lung, and Blood Research Review Committee B (HLBB)
Project Start
1993-08-01
Project End
1998-07-31
Budget Start
1994-08-01
Budget End
1995-07-31
Support Year
2
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
University of Maryland Baltimore
Department
Biochemistry
Type
Schools of Medicine
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201

  Publications

Bucci, Enrico; Pucciarelli, Stefania; Angeletti, Mauro (2013) Free energy changes and components implicit in the MWC allosteric model for the cooperative oxygen binding of hemoglobin. Biochemistry 52:4149-56
Koehler, Raymond C; Fronticelli, Clara; Bucci, Enrico (2008) Insensitivity of cerebral oxygen transport to oxygen affinity of hemoglobin-based oxygen carriers. Biochim Biophys Acta 1784:1387-94
Arosio, Daniele; Kwansa, Herman E; Gering, Henry et al. (2002) Static and dynamic light scattering approach to the hydration of hemoglobin and its supertetramers in the presence of osmolites. Biopolymers 63:1-11
Fronticelli, Clara; Bobofchak, Kevin M; Karavitis, Michael et al. (2002) Introduction of a new regulatory mechanism into human hemoglobin. Biophys Chem 98:115-26
Rebel, Annette; Ulatowski, John A; Joung, Karena et al. (2002) Regional cerebral blood flow in cats with cross-linked hemoglobin transfusion during focal cerebral ischemia. Am J Physiol Heart Circ Physiol 282:H832-41
Gattoni, M; Piro, M C; Boffi, A et al. (2001) The heme-globin and dimerization equilibria of recombinant human hemoglobins carrying site-specific beta chains mutations. Arch Biochem Biophys 386:172-8
Fronticelli, C; Arosio, D; Bobofchak, K M et al. (2001) Molecular engineering of a polymer of tetrameric hemoglobins. Proteins 44:212-22
Piro, M C; Militello, V; Leone, M et al. (2001) Heme pocket disorder in myoglobin: reversal by acid-induced soft refolding. Biochemistry 40:11841-50
Kwansa, H E; Young, A D; Arosio, D et al. (2000) Adipyl crosslinked bovine hemoglobins as new models of allosteric systems. Proteins 39:166-9
Matheson, B; Razynska, A; Kwansa, H et al. (2000) Appearance of dissociable and cross-linked hemoglobins in the renal hilar lymph. J Lab Clin Med 135:459-64

Showing the most recent 10 out of 42 publications