The Animal Core provides services to all four projects, since each project proposes to utilizemice as experimental models.Dr. Kylie Kavanagh, Core Leader, will oversee the duties of this core to assure that all neededanimal care is made readily available to each project. All aspects of surgery, postsurgical care andanimal husbandry will be coordinated through Dr. Kavanagh with the staff of the specific projectinvolved and assistance by Paul Sikoski, DVM, Director of the Animal Facility in BRF-1, and Dr. JanWagner, DVM Director of the WFUHS Animal Resources Program. Several post-DVM fellows as wellas three veterinary technicians will assist the veterinarians in care of program project animals.Surgical protocols have been utilized successfully during the last five year program project cycle afterbeing generated with the input of Dr. Rudel and veterinary staff and needed oversight is through theircollaborative efforts.In addition to surgery, technical services provided by the core staff will include blood collection,routine health measurements including regular body weight measurements, quantitative fecalcollections when required, and oversight of all breeding activities and housing needs in collaborationwith the animal care staff. A technician familiar with the experimental protocols related to eachproject has been assigned by project, and this technical staff member will be responsible for thespecific duties for that project. This group of technicians will benefit from interacting with Dr.Kavanagh and with other experienced staff members to make the husbandry aspects of the entireprogram project more efficient. In many cases, the diets to be fed to the mice are made especially forthe individual project, and the technical staff of the core will provide the liason function between theproject, the diet kitchen, and the Diet Analysis laboratory that provides quality control assays,assuring timely delivery of diet.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL049373-16
Application #
7537463
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2008-08-15
Project End
2013-06-30
Budget Start
2008-08-15
Budget End
2009-06-30
Support Year
16
Fiscal Year
2008
Total Cost
$353,951
Indirect Cost
Name
Wake Forest University Health Sciences
Department
Type
DUNS #
937727907
City
Winston-Salem
State
NC
Country
United States
Zip Code
27157
Schugar, Rebecca C; Shih, Diana M; Warrier, Manya et al. (2017) The TMAO-Producing Enzyme Flavin-Containing Monooxygenase 3 Regulates Obesity and the Beiging of White Adipose Tissue. Cell Rep 19:2451-2461
Pollard, Ricquita D; Fulp, Brian; Sorci-Thomas, Mary G et al. (2016) High-Density Lipoprotein Biogenesis: Defining the Domains Involved in Human Apolipoprotein A-I Lipidation. Biochemistry 55:4971-81
Rodríguez-Pérez, Celia; Ramprasath, Vanu Ramkumar; Pu, Shuaihua et al. (2016) Docosahexaenoic Acid Attenuates Cardiovascular Risk Factors via a Decline in Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Plasma Levels. Lipids 51:75-83
Warrier, Manya; Zhang, Jun; Bura, Kanwardeep et al. (2016) Sterol O-Acyltransferase 2-Driven Cholesterol Esterification Opposes Liver X Receptor-Stimulated Fecal Neutral Sterol Loss. Lipids 51:151-7
Jones, Peter J H; MacKay, Dylan S; Senanayake, Vijitha K et al. (2015) High-oleic canola oil consumption enriches LDL particle cholesteryl oleate content and reduces LDL proteoglycan binding in humans. Atherosclerosis 238:231-8
Lopez, Adam M; Chuang, Jen-Chieh; Posey, Kenneth S et al. (2015) PRD125, a potent and selective inhibitor of sterol O-acyltransferase 2 markedly reduces hepatic cholesteryl ester accumulation and improves liver function in lysosomal acid lipase-deficient mice. J Pharmacol Exp Ther 355:159-67
Liu, Mingxia; Allegood, Jeremy; Zhu, Xuewei et al. (2015) Uncleaved ApoM signal peptide is required for formation of large ApoM/sphingosine 1-phosphate (S1P)-enriched HDL particles. J Biol Chem 290:7861-70
Melchior, John T; Olson, John D; Kelley, Kathryn L et al. (2015) Targeted Knockdown of Hepatic SOAT2 With Antisense Oligonucleotides Stabilizes Atherosclerotic Plaque in ApoB100-only LDLr-/- Mice. Arterioscler Thromb Vasc Biol 35:1920-7
Ohshiro, Taichi; Ohtawa, Masaki; Nagamitsu, Tohru et al. (2015) New pyripyropene A derivatives, highly SOAT2-selective inhibitors, improve hypercholesterolemia and atherosclerosis in atherogenic mouse models. J Pharmacol Exp Ther 355:299-307
Marshall, Stephanie M; Gromovsky, Anthony D; Kelley, Kathryn L et al. (2014) Acute sterol o-acyltransferase 2 (SOAT2) knockdown rapidly mobilizes hepatic cholesterol for fecal excretion. PLoS One 9:e98953

Showing the most recent 10 out of 242 publications