Abstract

Hereditary and diet induced mouse and rabbit animal models for atherosclerosis will be used in this project. Baseline levels for hematologic and lipid chemistry parameters will be established and these data compared with those from animals receiving gene therapy. Dr. Montgomery's Comparative Pathology Laboratory will be responsible for collection of all blood samples from experimental animals. Plasma cholesterols and triglycerides and routine complete blood counts will be performed in his laboratory. Dr. Wolfgang Patsch's laboratory will conduct analyses for apolipoproteins A-I, E, and B utilizing immunoassays. Functional assays and/or immunoassays will be used to measure LDL receptors, 7 alpha-hydroxylase, and lipoprotein lipase in selected tissues or body fluids of control and experimental animals. This core lab will support three of the four research projects utilizing animals.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
1P01HL050422-01
Application #
3781172
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Type
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Waugh, J M; Li-Hawkins, J; Yuksel, E et al. (2000) Thrombomodulin overexpression to limit neointima formation. Circulation 102:332-7
Waugh, J M; Yuksel, E; Li, J et al. (1999) Local overexpression of thrombomodulin for in vivo prevention of arterial thrombosis in a rabbit model. Circ Res 84:84-92
Waugh, J M; Kattash, M; Li, J et al. (1999) Gene therapy to promote thromboresistance: local overexpression of tissue plasminogen activator to prevent arterial thrombosis in an in vivo rabbit model. Proc Natl Acad Sci U S A 96:1065-70
Rojas-Martinez, A; Wyde, P R; Montgomery, C A et al. (1998) Distribution, persistency, toxicity, and lack of replication of an E1A-deficient adenoviral vector after intracardiac delivery in the cotton rat. Cancer Gene Ther 5:365-70
Faith, R E; Montgomery, C A; Durfee, W J et al. (1997) The cotton rat in biomedical research. Lab Anim Sci 47:337-45
Gingras, M C; Arevalo, P; Aguilar-Cordova, E (1996) Potential salmon sperm origin of the E3 region insert of the adenovirus 5 dl309 mutant. Cancer Gene Ther 3:151-4
Sparrow, J T; Monera, O D (1996) Improvements to the TMSBr method of peptide resin deprotection and cleavage: application to large peptides. Pept Res 9:218-22
Gottschalk, S; Sparrow, J T; Hauer, J et al. (1996) A novel DNA-peptide complex for efficient gene transfer and expression in mammalian cells. Gene Ther 3:448-57
Guo, Z S; Wang, L H; Eisensmith, R C et al. (1996) Evaluation of promoter strength for hepatic gene expression in vivo following adenovirus-mediated gene transfer. Gene Ther 3:802-10
Gottschalk, S; Tweten, R K; Smith, L C et al. (1995) Efficient gene delivery and expression in mammalian cells using DNA coupled with perfringolysin O. Gene Ther 2:498-503

Showing the most recent 10 out of 13 publications