Abstract

This core will provide tissue culture, resources and/an adenovirus vector production facility to the Program Project. In addition, it will provide to the component projects freshly isolated human airway epithelial cells, and alveolar macrophages obtained by fiberoptic bronchoscopy. The tissue culture facility will provide a resource to bank various cell lines to be used by the Projects and Pilot/Feasibility Projects. It will also provide a site to culture cell lines, and the expertise to instruct investigators unfamiliar with tissue cultures in the relevant methods. The technical staff of the Core will receive human biologic specimens obtained by bronchoscopy, and prepare these samples for use by investigators of the component projects and CF-Pilot/Feasibility Projects. This core will be the major site of adenovirus vector production for the Program Project, including the cataloging and storage of various vectors, culturing of the 293 cell line used to produce the vectors, and the actual production and purification of the vectors. The core will be directed by R. Crystal, with use under the auspices and guidance of the Executive Committee.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL051746-04
Application #
5214113
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Ryan, Dorothy M; Vincent, Thomas L; Salit, Jacqueline et al. (2014) Smoking dysregulates the human airway basal cell transcriptome at COPD risk locus 19q13.2. PLoS One 9:e88051
Dvorak, Anna; Tilley, Ann E; Shaykhiev, Renat et al. (2011) Do airway epithelium air-liquid cultures represent the in vivo airway epithelium transcriptome? Am J Respir Cell Mol Biol 44:465-73
Krause, Anja; Whu, Wen Zhu; Xu, Yaqin et al. (2011) Protective anti-Pseudomonas aeruginosa humoral and cellular mucosal immunity by AdC7-mediated expression of the P. aeruginosa protein OprF. Vaccine 29:2131-9
Limberis, Maria P; Bell, Christie L; Heath, Jack et al. (2010) Activation of transgene-specific T cells following lentivirus-mediated gene delivery to mouse lung. Mol Ther 18:143-50
Song, Yuhu; Lou, Howard H; Boyer, Julie L et al. (2009) Functional cystic fibrosis transmembrane conductance regulator expression in cystic fibrosis airway epithelial cells by AAV6.2-mediated segmental trans-splicing. Hum Gene Ther 20:267-81
Vandenberghe, L H; Breous, E; Nam, H-J et al. (2009) Naturally occurring singleton residues in AAV capsid impact vector performance and illustrate structural constraints. Gene Ther 16:1416-28
Fein, David E; Limberis, Maria P; Maloney, Sean F et al. (2009) Cationic lipid formulations alter the in vivo tropism of AAV2/9 vector in lung. Mol Ther 17:2078-87
Limberis, Maria P; Vandenberghe, Luk H; Zhang, Liqun et al. (2009) Transduction efficiencies of novel AAV vectors in mouse airway epithelium in vivo and human ciliated airway epithelium in vitro. Mol Ther 17:294-301
Tertilt, Christine; Joh, Ju; Krause, Anja et al. (2009) Expression of B-cell activating factor enhances protective immunity of a vaccine against Pseudomonas aeruginosa. Infect Immun 77:3044-55
Limberis, M P; Bell, C L; Wilson, J M (2009) Identification of the murine firefly luciferase-specific CD8 T-cell epitopes. Gene Ther 16:441-7

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