Abstract

This Core, located in Vancouver, takes advantages of the specialized expertise and facilities established at the Terry Fox Laboratory for the in vitro and in vivo analysis of primitive hematopoietic cells of both mouse and human origin. The activities of the Core include: Production, backcrossing and testing of a large number and variety of inbred mice required for human and mouse stem cell assays and for evaluating gene therapy strategies in mouse models of thalassemia and Sickle Cell Anemia (SCA). 2) Procurement, processing and cryopreservation of cells from human adult bone marrow (BM) and steady-state and mobilized peripheral blood (PB), cord blood (CB) and fetal liver (FL) sources, including samples from thalassemic and SCA patients as well as normal individuals, and baboon BM. 3) Provision of in vitro (LTC-IC, CFC), and in vivo assays (CRU) for primitive human and murine hematopoietic cells for the testing of new vectors and gene transfer strategies. 4) Technology development-to improve the characterization and detection of human CRU and their in vivo generated erythroid progeny, and to develop procedures suitable for the large scale isolation of transduced stem cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL055435-07
Application #
6505101
Study Section
Project Start
2001-09-28
Project End
2002-08-31
Budget Start
Budget End
Support Year
7
Fiscal Year
2001
Total Cost
$266,631
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Dykstra, Brad; Kent, David; Bowie, Michelle et al. (2007) Long-term propagation of distinct hematopoietic differentiation programs in vivo. Cell Stem Cell 1:218-29
Bowie, Michelle B; Kent, David G; Dykstra, Brad et al. (2007) Identification of a new intrinsically timed developmental checkpoint that reprograms key hematopoietic stem cell properties. Proc Natl Acad Sci U S A 104:5878-82
Bowie, Michelle B; Kent, David G; Copley, Michael R et al. (2007) Steel factor responsiveness regulates the high self-renewal phenotype of fetal hematopoietic stem cells. Blood 109:5043-8
Dykstra, Brad; Ramunas, John; Kent, David et al. (2006) High-resolution video monitoring of hematopoietic stem cells cultured in single-cell arrays identifies new features of self-renewal. Proc Natl Acad Sci U S A 103:8185-90
Olivier, Emmanuel N; Rybicki, Anne C; Bouhassira, Eric E (2006) Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells. Stem Cells 24:1914-22
Peshkovsky, Alexey; Kennan, Richard P; Nagel, Ronald L et al. (2006) Sensitivity enhancement and compensation of RF penetration artifact with planar actively detunable quadrature surface coil. Magn Reson Imaging 24:81-7
Bowie, Michelle B; McKnight, Kristen D; Kent, David G et al. (2006) Hematopoietic stem cells proliferate until after birth and show a reversible phase-specific engraftment defect. J Clin Invest 116:2808-16
Fu, Haiqing; Wang, Lixin; Lin, Chii-Mei et al. (2006) Preventing gene silencing with human replicators. Nat Biotechnol 24:572-6
Fischer, Marlene; Schmidt, Manfred; Klingenberg, Silke et al. (2006) Short-term repopulating cells with myeloid potential in human mobilized peripheral blood do not have a side population (SP) phenotype. Blood 108:2121-3
Dykxhoorn, Derek M; Schlehuber, Lisa D; London, Irving M et al. (2006) Determinants of specific RNA interference-mediated silencing of human beta-globin alleles differing by a single nucleotide polymorphism. Proc Natl Acad Sci U S A 103:5953-8

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