The Gene Expression & Molecular Genetics Core (B) is responsible for molecular biology support and cloning/expression studies as well as providing reagents to member projects. The types of services and supplies needed will, of course, vary between projects; however, the overall goal of the GEMG Core is to ensure that the members have the reagent, techniques and assistance needed to perform these studies. 1. Standardized reagents - enzymes, primers, PCR master mixes, probes. 2. Banking of plasmids, vectors, RNA from synchronized cells, etc. 3. In Situ PCR and RT=PCR 4. Establishing producer/packaging cell lines 5. Viral titer, supernatant harvest and storage 6. Validation assays - thymidine uptake, colony RT-PCR, colony PCR 7. Additional manpower for large procedures, e.g. transplants
| Dooner, Gerri J; Colvin, Gerald A; Dooner, Mark S et al. (2008) Gene expression fluctuations in murine hematopoietic stem cells with cell cycle progression. J Cell Physiol 214:786-95 |
| Colvin, Gerald A; Lambert, Jean-Francois; Dooner, Mark S et al. (2007) Murine allogeneic in vivo stem cell homing(,). J Cell Physiol 211:386-91 |
| Kieusseian, Aurelie; Chagraoui, Jalila; Kerdudo, Cecile et al. (2006) Expression of Pitx2 in stromal cells is required for normal hematopoiesis. Blood 107:492-500 |
| Aliotta, Jason M; Keaney, Patrick; Passero, Michael et al. (2006) Bone marrow production of lung cells: the impact of G-CSF, cardiotoxin, graded doses of irradiation, and subpopulation phenotype. Exp Hematol 34:230-41 |
| Zhang, Hui Z; Degar, Barbara A; Rogoulina, Svetlana et al. (2006) Hematopoiesis following disruption of the Pitx2 homeodomain gene. Exp Hematol 34:167-78 |
| Abedi, Mehrdad; Greer, Deborah A; Foster, Bethany M et al. (2005) Critical variables in the conversion of marrow cells to skeletal muscle. Blood 106:1488-94 |
| Quesenberry, Peter J; Colvin, Gerald; Abedi, Mehrdad (2005) Perspective: fundamental and clinical concepts on stem cell homing and engraftment: a journey to niches and beyond. Exp Hematol 33:9-19 |
| Quesenberry, Peter J; Colvin, Gerald A; Abedi, Mehrdad et al. (2005) The stem cell continuum. Ann N Y Acad Sci 1044:228-35 |
| Colvin, Gerald A; Lambert, Jean-Francois; Abedi, M et al. (2004) Differentiation hotspots: the deterioration of hierarchy and stochasm. Blood Cells Mol Dis 32:34-41 |
| Nowakowski, Grzegorz S; Dooner, Mark S; Valinski, Helen M et al. (2004) A specific heptapeptide from a phage display peptide library homes to bone marrow and binds to primitive hematopoietic stem cells. Stem Cells 22:1030-8 |
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