Abstract

The DNA Core provides DNA oligonucleotide synthesis and DNA sequencing services for the projects described in this proposal. The Core will provide rapid, high-quality, automated DNA Sequencing using a cycled sequencing protocol. Sequence analysis will be performed on a variety of DAN templates and customized to the needs of each investigator. This important technological support should be of major value to all PPG projects. Through the mechanisms of this DNA core within the PPG program, a major discount and resulting savings in operating expenses will be achieved. All four projects are also heavily dependent on oligonucleotide synthesis service. By coordinating oligonucleotide purchases through the core budget, a significant discount has been offered through the university of Michigan DAN Synthesis Laboratory. The DNA Synthesis Laboratory will provide routine 40 nanomole or 200 nanomole syntheses with a normal delivery time of two working days after receipt of orders. Oligonucleotide quality is assured through monitoring trityls as well as analyses of oligonucleotides by reverse- phased HPLC. Specialized custom synthesis chemistries are also available.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL057346-02
Application #
6110822
Study Section
Project Start
1998-09-01
Project End
1999-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Michigan Ann Arbor
Department
Type
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109

  Publications

Tomberg, Kärt; Westrick, Randal J; Kotnik, Emilee N et al. (2018) Whole exome sequencing of ENU-induced thrombosis modifier mutations in the mouse. PLoS Genet 14:e1007658
Khoriaty, Rami; Hesketh, Geoffrey G; Bernard, Amélie et al. (2018) Functions of the COPII gene paralogs SEC23A and SEC23B are interchangeable in vivo. Proc Natl Acad Sci U S A 115:E7748-E7757
Nie, Chao; Wang, Huimin; Wang, Rui et al. (2018) Dimeric sorting code for concentrative cargo selection by the COPII coat. Proc Natl Acad Sci U S A 115:E3155-E3162
Ji, Y; Adeola, O; Strawn, T L et al. (2017) Recombinant soluble apyrase APT102 inhibits thrombosis and intimal hyperplasia in vein grafts without adversely affecting hemostasis or re-endothelialization. J Thromb Haemost 15:814-825
Westrick, Randal J; Tomberg, Kärt; Siebert, Amy E et al. (2017) Sensitized mutagenesis screen in Factor V Leiden mice identifies thrombosis suppressor loci. Proc Natl Acad Sci U S A 114:9659-9664
Khoriaty, Rami; Vogel, Nancy; Hoenerhoff, Mark J et al. (2017) SEC23B is required for pancreatic acinar cell function in adult mice. Mol Biol Cell 28:2146-2154
Ji, Yan; Weng, Zhen; Fish, Philip et al. (2016) Pharmacological Targeting of Plasminogen Activator Inhibitor-1 Decreases Vascular Smooth Muscle Cell Migration and Neointima Formation. Arterioscler Thromb Vasc Biol 36:2167-2175
Khoriaty, Rami; Everett, Lesley; Chase, Jennifer et al. (2016) Pancreatic SEC23B deficiency is sufficient to explain the perinatal lethality of germline SEC23B deficiency in mice. Sci Rep 6:27802
Khoobchandani, Menka; Katti, Kavita; Maxwell, Adam et al. (2016) Laminin Receptor-Avid Nanotherapeutic EGCg-AuNPs as a Potential Alternative Therapeutic Approach to Prevent Restenosis. Int J Mol Sci 17:316
Xu, Xianjin; Ma, Zhiwei; Sun, Hongmin et al. (2016) SM-TF: A structural database of small molecule-transcription factor complexes. J Comput Chem 37:1559-64

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