Abstract

The protein expression core is designed to continue to provide highly purified recombinant proteins to eachof the component projects. The core will provide advice about preparing cDNA expression constructs.Prokaryotic expression will be achieved primarily by use of vectors such as PET-15 or as fusion proteins withglutathione S-transferase or maltose-binding protein using vectors such as pGEX or pMAL. In selectedcases, expression in insect cells will be employed to produce recombinant Fab fragments or when criticalpost-translational modifications are required for activity. The core will perform microbiological manipulationsand fermentations. Proteins will be purified by affinity chromatography and fast protein and high performanceliquid chromatography and chemically characterized by mass spectroscopy. The core will also preparespecialized novel model protein mimics of integrin cytoplasmic domains for analysis of interactions at thecytoplasmic face of these receptors

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL057900-12A1
Application #
7425543
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2007-12-01
Project End
2012-11-30
Budget Start
2007-12-01
Budget End
2009-01-31
Support Year
12
Fiscal Year
2008
Total Cost
$155,262
Indirect Cost

  Institution

Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093

  Publications

Wilson, RaeAnna; Espinosa-Diez, Cristina; Kanner, Nathan et al. (2016) MicroRNA regulation of endothelial TREX1 reprograms the tumour microenvironment. Nat Commun 7:13597
Liao, Zhongji; Kato, Hisashi; Pandey, Manjula et al. (2015) Interaction of kindlin-2 with integrin ?3 promotes outside-in signaling responses by the ?V?3 vitronectin receptor. Blood 125:1995-2004
Desgrosellier, Jay S; Lesperance, Jacqueline; Seguin, Laetitia et al. (2014) Integrin ?v?3 drives slug activation and stemness in the pregnant and neoplastic mammary gland. Dev Cell 30:295-308
Fitzpatrick, Paul; Shattil, Sanford J; Ablooglu, Ararat J (2014) C-terminal COOH of integrin ?1 is necessary for ?1 association with the kindlin-2 adapter protein. J Biol Chem 289:11183-93
Seguin, Laetitia; Kato, Shumei; Franovic, Aleksandra et al. (2014) An integrin ??-KRAS-RalB complex drives tumour stemness and resistance to EGFR inhibition. Nat Cell Biol 16:457-68
Casar, B; Rimann, I; Kato, H et al. (2014) In vivo cleaved CDCP1 promotes early tumor dissemination via complexing with activated ?1 integrin and induction of FAK/PI3K/Akt motility signaling. Oncogene 33:255-68
Ye, Feng; Petrich, Brian G; Anekal, Praju et al. (2013) The mechanism of kindlin-mediated activation of integrin ?IIb?3. Curr Biol 23:2288-2295
Scheppke, Lea; Murphy, Eric A; Zarpellon, Alessandro et al. (2012) Notch promotes vascular maturation by inducing integrin-mediated smooth muscle cell adhesion to the endothelial basement membrane. Blood 119:2149-58
Cantor, Joseph M; Ginsberg, Mark H (2012) CD98 at the crossroads of adaptive immunity and cancer. J Cell Sci 125:1373-82
Banno, Asoka; Goult, Benjamin T; Lee, HoSup et al. (2012) Subcellular localization of talin is regulated by inter-domain interactions. J Biol Chem 287:13799-812

Showing the most recent 10 out of 79 publications