The purpose of the Tissue Culture Core is to provide cells, tissue culture services, and technical advice to the investigators of projects 1-3. The function of the core can be summarized in the following specific aims: 1. To provide a supply of smooth muscle cells and if necessary endothelial cells and monocytes to PPG investigators. 2. To prepare primary cultures of rat aortic smooth muscle cells (RASM) and human saphenous vein smooth muscle cells (HSVSMC) for amplification and passage. 3. To provide quality and cost control through bulk purchases of tissue and cell culture reagents and through routine screening of cell lines for contamination. 4. To provide training to PPG investigators regarding techniques of cell culture and isolation.
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| Kim, C; Fang, F; Weyand, C M et al. (2017) The life cycle of a T cell after vaccination - where does immune ageing strike? Clin Exp Immunol 187:71-81 |
| Foss, Jason D; Kirabo, Annet; Harrison, David G (2017) Do high-salt microenvironments drive hypertensive inflammation? Am J Physiol Regul Integr Comp Physiol 312:R1-R4 |
| Loperena, Roxana; Harrison, David G (2017) Oxidative Stress and Hypertensive Diseases. Med Clin North Am 101:169-193 |
| Weyand, Cornelia M; Zeisbrich, Markus; Goronzy, Jörg J (2017) Metabolic signatures of T-cells and macrophages in rheumatoid arthritis. Curr Opin Immunol 46:112-120 |
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| Montaniel, Kim Ramil C; Harrison, David G (2016) Is Hypertension a Bone Marrow Disease? Circulation 134:1369-1372 |
| Wen, Zhenke; Shimojima, Yasuhiro; Shirai, Tsuyoshi et al. (2016) NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs. J Clin Invest 126:1953-67 |
| Watanabe, Ryu; Goronzy, Jörg J; Berry, Gerald et al. (2016) Giant Cell Arteritis: From Pathogenesis to Therapeutic Management. Curr Treatm Opt Rheumatol 2:126-137 |
| Qi, Qian; Cavanagh, Mary M; Le Saux, Sabine et al. (2016) Diversification of the antigen-specific T cell receptor repertoire after varicella zoster vaccination. Sci Transl Med 8:332ra46 |
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