Abstract

The tissue culture core in this program project will provide a centralized culture facility to support the four research projects investigating endothelial cell (EC) carrier dysfunction. Each of the four projects will utilize four or more of the six cultured endothelial cell types and/or cultured smooth muscle cells. Specifically, the cell culture core will provide freshly isolated and cultured human umbilical vein endothelial cells, both freshly isolated and cell lines of bovine pulmonary artery smooth muscle cells, and freshly isolated human neutrophils. The extensive experience of the tissue culture core personnel will assure the continuous supply of high quality cells that are fully characterized for their purity. The grouping of the tissue culture facilities in a core function will also provide the most economical growth of cells which are maintained in numbers adequate to supply all of the investigators of the program project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL058064-03
Application #
6110843
Study Section
Project Start
1999-02-01
Project End
2000-01-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Wang, Ting; Brown, Mary E; Kelly, Gabriel T et al. (2018) Myosin light chain kinase ( MYLK) coding polymorphisms modulate human lung endothelial cell barrier responses via altered tyrosine phosphorylation, spatial localization, and lamellipodial protrusions. Pulm Circ 8:2045894018764171
Wang, X; Wang, L; Garcia, J G N et al. (2018) The Significant Role of c-Abl Kinase in Barrier Altering Agonists-mediated Cytoskeletal Biomechanics. Sci Rep 8:1002
Oita, Radu C; Camp, Sara M; Ma, Wenli et al. (2018) Novel Mechanism for Nicotinamide Phosphoribosyltransferase Inhibition of TNF-?-mediated Apoptosis in Human Lung Endothelial Cells. Am J Respir Cell Mol Biol 59:36-44
Szilágyi, Keely L; Liu, Cong; Zhang, Xu et al. (2017) Epigenetic contribution of the myosin light chain kinase gene to the risk for acute respiratory distress syndrome. Transl Res 180:12-21
Wang, X; Bleher, R; Wang, L et al. (2017) Imatinib Alters Agonists-mediated Cytoskeletal Biomechanics in Lung Endothelium. Sci Rep 7:14152
Shekhawat, Gajendra S; Dudek, Steven M; Dravid, Vinayak P (2017) Development of ultrasound bioprobe for biological imaging. Sci Adv 3:e1701176
Mascarenhas, Joseph B; Tchourbanov, Alex Y; Fan, Hanli et al. (2017) Mechanical Stress and Single Nucleotide Variants Regulate Alternative Splicing of the MYLK Gene. Am J Respir Cell Mol Biol 56:29-37
Belvitch, Patrick; Brown, Mary E; Brinley, Brittany N et al. (2017) The ARP 2/3 complex mediates endothelial barrier function and recovery. Pulm Circ 7:200-210
Tao, Shasha; Rojo de la Vega, Montserrat; Quijada, Hector et al. (2016) Bixin protects mice against ventilation-induced lung injury in an NRF2-dependent manner. Sci Rep 6:18760
Viswanathan, P; Ephstein, Y; Garcia, J G N et al. (2016) Differential elastic responses to barrier-altering agonists in two types of human lung endothelium. Biochem Biophys Res Commun 478:599-605

Showing the most recent 10 out of 270 publications