The principal objective of the Biophysical Imaging Core is to provide Program investigators with a completerange of expertise, training, equipments, and data analysis tools to obtain nano-to-micro scale biophysicalinformation pertaining to the cellular and molecular basis of endothelial barrier permeability. Core Dpersonnel and equipment will allow PPG scientists to image 3D structures, evaluate physical and chemicalproperties and define perturbant-induced real-time changes in the structures and activity of cells andsubcellular constituents, including membranes, cytoskeletal networks and cell-matrix and cell-cell junctions.This Biolmaging Core supports the PPG's five research projects with quantitative microscopy related toatomic force microscopy (AFM), light fluorescence microscopy and TIRF (total internal reflectionfluorescence microscopy). It offers access to experienced use of the complete resources of the Center forNanomedicine that includes state-of-the-art atomic force microscopes integrated with high resolution singlephoton microscopy and fluorescence microscopy systems for simultaneous multimodal correlative studies. Inaddition, this Core will also make use of the common resources available at the University of Chicago IBD(Institute for Biophysical Dynamics) Microscope Facility (which includes an excellent electron microscopefacility) and the Department of Medicine's Multiphoton Laser Scanning Microscope Facility. Led by CoreLeader Ratnesh Lai, PhD, the core has assembled the personnel and laboratory facilities to satisfy a widerange of experimental 3D imaging and mechanobiophysics needs. Core D personnel have professionalexperience spanning the fields of high resolution imaging and examining physical and chemical properties,including mechanobiophysics with various scanning probe microscopies. Despite relative recent arrival ofCore personnel within the University of Chicago, these talented scientists have provided convincingpreliminary data for each Project and all five research projects.
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