This Program Project represents a cohesive group of laboratories and investigators that share common goals and strategies aimed at transferring genes to progenitor and differentiated cells. These laboratories also share a need for several techniques such as the creation of gene transfer vectors and the analysis of data generated by gene transfer studies. The Cell Biology Core Facility aims to provide this group of investigators with a variety of imaging and detection methods to show interaction of vectors with target cells, expression of vector-encoded transgenes, and the physiological impact of transgene expression. Toward this end, the Cell Biology Core FaCility maintains state-of-the-art equipment for fluorescence detection, optimal filters and documentation equipment for brightfield and fluorescence image acquisition, and facilitated access to additional medical Center resources such as histological, ultrastructural, and con focal facilities. Furthermore, the staff of the Cell Biology Core Facility has extensive training in a variety of cytological and histological techniques and will dedicate time and resources to make those assets available to the participating Projects and Pilots. The goal of this Core Facility as well as the other core facilities associated with the Program Project will be realized in making the whole of this research endeavor greater than the sum of its parts.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL059312-02
Application #
6110882
Study Section
Project Start
1998-09-28
Project End
1999-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Weill Medical College of Cornell University
Department
Type
DUNS #
201373169
City
New York
State
NY
Country
United States
Zip Code
10065
Ding, Bi-Sen; Nolan, Daniel J; Butler, Jason M et al. (2010) Inductive angiocrine signals from sinusoidal endothelium are required for liver regeneration. Nature 468:310-5
Kobayashi, Hideki; Butler, Jason M; O'Donnell, Rebekah et al. (2010) Angiocrine factors from Akt-activated endothelial cells balance self-renewal and differentiation of haematopoietic stem cells. Nat Cell Biol 12:1046-56
Rabbany, Sina Y; James, Daylon; Rafii, Shahin (2010) New dimensions in vascular engineering: opportunities for cancer biology. Tissue Eng Part A 16:2157-9
Rafii, Shahin; Nolan, Daniel (2010) Cholesterol activates vascular niche and hematopoiesis. Blood 115:3857-8
Yamamoto, Masaya; James, Daylon; Li, Hui et al. (2010) Generation of stable co-cultures of vascular cells in a honeycomb alginate scaffold. Tissue Eng Part A 16:299-308
Butler, Jason M; Kobayashi, Hideki; Rafii, Shahin (2010) Instructive role of the vascular niche in promoting tumour growth and tissue repair by angiocrine factors. Nat Rev Cancer 10:138-46
Butler, Jason M; Nolan, Daniel J; Vertes, Eva L et al. (2010) Endothelial cells are essential for the self-renewal and repopulation of Notch-dependent hematopoietic stem cells. Cell Stem Cell 6:251-64
James, Daylon; Nam, Hyung-song; Seandel, Marco et al. (2010) Expansion and maintenance of human embryonic stem cell-derived endothelial cells by TGFbeta inhibition is Id1 dependent. Nat Biotechnol 28:161-6
Kiuru, Maija; Hidaka, Chisa; Hubner, Ralf-Harto et al. (2009) Sonic hedgehog expands diaphyseal trabecular bone altering bone marrow niche and lymphocyte compartment. Mol Ther 17:1442-52
Hooper, Andrea T; Shmelkov, Sergey V; Gupta, Sunny et al. (2009) Angiomodulin is a specific marker of vasculature and regulates vascular endothelial growth factor-A-dependent neoangiogenesis. Circ Res 105:201-8

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