Abstract

Biochemical core lab of this program project grant will provide a consolidated highly specialized well-equipped and professional staff unit capable of performing a variety of radio-immunoassay, biochemical and molecular procedures as required by project investigators. No single investigator laboratory supported with R01 support alone would be capable of equipping and staffing this analytical resource or personnel who will analyze samples for P450 and NOS activities using radiochemical HPLC assay measurements, 20-HETE and EETs in CSF microdialysis efficient using new fluorescent HPLC assays. This core will also measure IP3, DAG, microglial and estroglial products in the brain and cerebrovasculature. In addition, we will measure P4504 and NOS protein using immunoblot and measure molecular measurements with competitive RCPR. Access to the analytical expertise and equipment in the central facility is essential to each of the proposed projects and justified by the large number of biochemical and molecular determinations required for the proposed studies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL059996-02
Application #
6302510
Study Section
Project Start
2000-04-01
Project End
2001-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
2
Fiscal Year
2000
Total Cost
$339,485
Indirect Cost

  Institution

Name
Medical College of Wisconsin
Department
Type
DUNS #
073134603
City
Milwaukee
State
WI
Country
United States
Zip Code
53226

  Publications

Gebremedhin, Debebe; Zhang, David X; Carver, Koryn A et al. (2016) Expression of CYP 4A ?-hydroxylase and formation of 20-hydroxyeicosatetreanoic acid (20-HETE) in cultured rat brain astrocytes. Prostaglandins Other Lipid Mediat 124:16-26
Pabbidi, Mallikarjuna R; Mazur, Olga; Fan, Fan et al. (2014) Enhanced large conductance K+ channel activity contributes to the impaired myogenic response in the cerebral vasculature of Fawn Hooded Hypertensive rats. Am J Physiol Heart Circ Physiol 306:H989-H1000
Gebremedhin, Debebe; Gopalakrishnan, Sandeep; Harder, David R (2014) Endogenous events modulating myogenic regulation of cerebrovascular function. Curr Vasc Pharmacol 12:810-7
Carver, Koryn A; Lourim, David; Tryba, Andrew K et al. (2014) Rhythmic expression of cytochrome P450 epoxygenases CYP4x1 and CYP2c11 in the rat brain and vasculature. Am J Physiol Cell Physiol 307:C989-98
Gebremedhin, Debebe; Terashvili, Maia; Wickramasekera, Nadi et al. (2013) Redox signaling via oxidative inactivation of PTEN modulates pressure-dependent myogenic tone in rat middle cerebral arteries. PLoS One 8:e68498
Wickramasekera, Nadi T; Gebremedhin, Debebe; Carver, Koryn A et al. (2013) Role of dual-specificity protein phosphatase-5 in modulating the myogenic response in rat cerebral arteries. J Appl Physiol 114:252-61
Yang, Zeng-Jin; Carter, Erin L; Kibler, Kathleen K et al. (2012) Attenuation of neonatal ischemic brain damage using a 20-HETE synthesis inhibitor. J Neurochem 121:168-79
Renic, Marija; Kumar, Suresh N; Gebremedhin, Debebe et al. (2012) Protective effect of 20-HETE inhibition in a model of oxygen-glucose deprivation in hippocampal slice cultures. Am J Physiol Heart Circ Physiol 302:H1285-93
Liu, Xiaoguang; Li, Chunyuan; Falck, John R et al. (2012) Relative contribution of cyclooxygenases, epoxyeicosatrienoic acids, and pH to the cerebral blood flow response to vibrissal stimulation. Am J Physiol Heart Circ Physiol 302:H1075-85
Terashvili, M; Sarkar, P; Nostrand, M V et al. (2012) The protective effect of astrocyte-derived 14,15-epoxyeicosatrienoic acid on hydrogen peroxide-induced cell injury in astrocyte-dopaminergic neuronal cell line co-culture. Neuroscience 223:68-76

Showing the most recent 10 out of 62 publications