The overall goal of the Transgenic Animal/Recombinant Protein Core Laboratory is to undertake and coordinate the breeding/cross-breeding of transgenic mice, and to prepare large amounts or recombinant proteins (sRAGE, sVCAM,-1, and RAGE-poliovirus receptor chimeras) for use in the proposed studies. Mouse breeding takes place in the Institute of Comparative Medicine of Columbia University, on the eighteenth floor of the P&S buildings (one floor above the laboratories where all three projects will be performed). Preparation of recombinant proteins is undertaken in laboratories of the investigators on the PPG) where there are tissue culture facilities for growing Sf9 cells and for performing infections with baculovirus constructs, as well as equipment for protein purification on an analytic and preparative scale (HPLC, FPLC, Isoelectric focussing, Smart and conventional chromatographic systems). Drs. Schmidt and Costantini, with the assistance of Dr. Yemul, will be responsible for breeding of transgenic mice. Dr. Schmidt, who discovered RAGE, is an expert in protein chemistry and will also supervise Dr. S. Yemul in the expression and purification of sRAGE, sVCAM-1 and related molecules. Each will be produced using the baculovirus system, and purified by methods which Dr. Schmidt has already worked out. In addition, Drs. Schmidt and Yemul will prepare and characterize AGE-modified proteins for studies proposed in the projects. This Core Laboratory will be utilized by each of the three components of the PPG.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Columbia University (N.Y.)
New York
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Schmidt, Ann Marie (2018) Highlighting Diabetes Mellitus: The Epidemic Continues. Arterioscler Thromb Vasc Biol 38:e1-e8
Lee, Gloria; Plaksin, Joseph; Ramasamy, Ravichandran et al. (2018) Targeted drug discovery and development, from molecular signaling to the global market: an educational program at New York University, 5-year metrics. J Transl Sci 4:1-9
Lee, Gloria; Kranzler, Jay D; Ramasamy, Ravichandran et al. (2018) Training scientists as future industry leaders: teaching translational science from an industry executive's perspective. J Transl Sci 4:
Schmidt, Ann Marie (2017) 2016ATVBPlenary Lecture: Receptor for Advanced Glycation Endproducts and Implications for the Pathogenesis an Treatment of Cardiometabolic Disorders: Spotlight on the Macrophage. Arterioscler Thromb Vasc Biol 37:613-621
López-Díez, Raquel; Shen, Xiaoping; Daffu, Gurdip et al. (2017) Ager Deletion Enhances Ischemic Muscle Inflammation, Angiogenesis, and Blood Flow Recovery in Diabetic Mice. Arterioscler Thromb Vasc Biol 37:1536-1547
Shekhtman, Alexander; Ramasamy, Ravichandran; Schmidt, Ann Marie (2017) Glycation & the RAGE axis: targeting signal transduction through DIAPH1. Expert Rev Proteomics 14:147-156
Senatus, Laura M; Schmidt, Ann Marie (2017) The AGE-RAGE Axis: Implications for Age-Associated Arterial Diseases. Front Genet 8:187
Thiagarajan, Devi; Ananthakrishnan, Radha; Zhang, Jinghua et al. (2016) Aldose Reductase Acts as a Selective Derepressor of PPAR? and the Retinoic Acid Receptor. Cell Rep 15:181-196
Ramasamy, Ravichandran; Shekhtman, Alexander; Schmidt, Ann Marie (2016) The multiple faces of RAGE--opportunities for therapeutic intervention in aging and chronic disease. Expert Opin Ther Targets 20:431-46
López-Díez, Raquel; Shekhtman, Alexander; Ramasamy, Ravichandran et al. (2016) Cellular mechanisms and consequences of glycation in atherosclerosis and obesity. Biochim Biophys Acta 1862:2244-2252

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