Abstract

of the core unit. In support of the experiments described in projects 1-4, the overall function of Core B will be to facilitate the identification, quantification, and analysis of cells either in tissue sections or in suspension. The accomplish this, Core B will provide the facilities methodology and expertise for flow cytometric, histologic and immunohistologic analyses. In addition to providing analytic services, Core B will also produce a range of purified and/or conjugated antibodies. These reagents will support not only FACS and histology (all projects), but will also be utilized for in vivo blocking (all projects), in vivo and in vitro cellular histology (all projects), but will also be utilized for in vivo blocking (all projects), in vivo and in vitro cellular depletion and ELISA techniques.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL063925-05
Application #
6995199
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2004-12-01
Budget End
2005-11-30
Support Year
5
Fiscal Year
2005
Total Cost
$118,675
Indirect Cost

  Institution

Name
Trudeau Institute, Inc.
Department
Type
DUNS #
020658969
City
Saranac Lake
State
NY
Country
United States
Zip Code
12983

  Publications

Ely, Kenneth H; Roberts, Alan D; Kohlmeier, Jacob E et al. (2007) Aging and CD8+ T cell immunity to respiratory virus infections. Exp Gerontol 42:427-31
Wiley, James A; Tighe, Michael P; Harmsen, Allen G (2005) Upper respiratory tract resistance to influenza infection is not prevented by the absence of either nasal-associated lymphoid tissue or cervical lymph nodes. J Immunol 175:3186-96
Powell, Timothy J; Brown, Deborah M; Hollenbaugh, Joseph A et al. (2004) CD8+ T cells responding to influenza infection reach and persist at higher numbers than CD4+ T cells independently of precursor frequency. Clin Immunol 113:89-100
Brown, Deborah M; Roman, Eulogia; Swain, Susan L (2004) CD4 T cell responses to influenza infection. Semin Immunol 16:171-7
Cauley, Linda S; Cookenham, Tres; Hogan, Robert J et al. (2003) Renewal of peripheral CD8+ memory T cells during secondary viral infection of antibody-sufficient mice. J Immunol 170:5597-606
Woodland, David L; Dutton, Richard W (2003) Heterogeneity of CD4(+) and CD8(+) T cells. Curr Opin Immunol 15:336-42
Blackman, Marcia A; Rouse, Barry T; Chisari, Frank V et al. (2002) Viral immunology: challenges associated with the progression from bench to clinic. Trends Immunol 23:565-7
Arnold, Paula Y; Vignali, Kate M; Miller, Timothy B et al. (2002) Reliable generation and use of MHC class II:gamma2aFc multimers for the identification of antigen-specific CD4(+) T cells. J Immunol Methods 271:137-51
Cauley, Linda S; Hogan, Robert J; Woodland, David L (2002) Memory T-cells in non-lymphoid tissues. Curr Opin Investig Drugs 3:33-6
Wiley, J A; Hogan, R J; Woodland, D L et al. (2001) Antigen-specific CD8(+) T cells persist in the upper respiratory tract following influenza virus infection. J Immunol 167:3293-9