Abstract

The purpose of this core unit is to utilize discarded vascular material at Brigham and Women's Hospital in order to provide primary human vascular cells for Projects 1, 2, and 3 as well as rat cardiomyocytes for Project 4. Through a dedicated cell culture technician, we will obtain human saphenous and human aortic tissue (excess tissue from orthotopic hear and Women's Hospital and provide cells to the projects at MIT; this will be much more efficient than individual procurement of tissues by investigators at MIT. Dr. Lee's laboratory is in the Thorn Research Building, attached to the main hospital building and allowing rapid access to these discarded tissues.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL064858-05
Application #
7124329
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2005-09-01
Budget End
2006-08-31
Support Year
5
Fiscal Year
2005
Total Cost
$249,206
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Rahim, Nur Aida Adbul; Pelet, Serge; Kamm, Roger D et al. (2012) Methodological considerations for global analysis of cellular FLIM/FRET measurements. J Biomed Opt 17:026013
Chung, Euiheon; Kim, Yang-Hyo; Tang, Wai Teng et al. (2009) Wide-field extended-resolution fluorescence microscopy with standing surface-plasmon-resonance waves. Opt Lett 34:2366-8
Jonas, Maxine; Huang, Hayden; Kamm, Roger D et al. (2008) Fast fluorescence laser tracking microrheometry. I: instrument development. Biophys J 94:1459-69
Jonas, Maxine; Huang, Hayden; Kamm, Roger D et al. (2008) Fast fluorescence laser tracking microrheometry, II: quantitative studies of cytoskeletal mechanotransduction. Biophys J 95:895-909
Ferrer, Jorge M; Lee, Hyungsuk; Chen, Jiong et al. (2008) Measuring molecular rupture forces between single actin filaments and actin-binding proteins. Proc Natl Acad Sci U S A 105:9221-6
Previte, Michael J R; Pelet, Serge; Kim, Ki Hean et al. (2008) Spectrally resolved fluorescence correlation spectroscopy based on global analysis. Anal Chem 80:3277-84
Lee, S E; Chunsrivirot, S; Kamm, R D et al. (2008) Molecular dynamics study of talin-vinculin binding. Biophys J 95:2027-36
Jonas, Maxine; So, Peter T C; Huang, Hayden (2008) Cell mechanics at multiple scales. Methods Enzymol 443:177-98
Norton, G V; Novarini, J C (2007) Modeling ultrasonic transient scattering from biological tissues including their dispersive properties directly in the time domain. Mol Cell Biomech 4:75-85
Su, Judith; Jiang, Xingyu; Welsch, Roy et al. (2007) Geometric confinement influences cellular mechanical properties I -- adhesion area dependence. Mol Cell Biomech 4:87-104

Showing the most recent 10 out of 43 publications