Abstract

The goal of the animal core facility is to support the experimental efforts of the different investigators of this proposal, by providing them with a reliably supply of well-characterized. The mice that this core will breed and/or maintain will include mice genetically deficient in fVIII, due to a targeted disruption of either exon 16 or exon 17 of the fVIII gene (henceforth referred to as X-16 and X-17 mice); double mutant mice carrying a disruption of both the fVIII gene and of the gene of either interleukin-4 (IL-4) or IL-12; and wild-type C57Bl/6 mice, to serve as controls. Random samples of the mice will be routinely checked to ensure absence of wild-type genes in the strains carrying disruptions of the fVIII or cytokine genes. This will be done by PCR of blood samples of the individual mice, using suitable primers. Also, we plan to identify any presence of truncated fVIII products in X-16 and X-17 mice using Western blots of mouse blood and sequence-specific anti-fVIII antisera.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL065578-03
Application #
6642376
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2002-08-01
Project End
2003-07-31
Budget Start
Budget End
Support Year
3
Fiscal Year
2002
Total Cost
$267,358
Indirect Cost

  Institution

Name
University of Minnesota Twin Cities
Department
Type
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455

  Publications

Gharagozlou, Soheila; Sharifian, Ramazan A; Khoshnoodi, Jalal et al. (2009) Epitope specificity of anti-factor VIII antibodies from inhibitor positive acquired and congenital haemophilia A patients using synthetic peptides spanning A and C domains. Thromb Haemost 101:834-9
Hu, Genlin; Guo, Delan; Key, Nigel S et al. (2007) Cytokine production by CD4+ T cells specific for coagulation factor VIII in healthy subjects and haemophilia A patients. Thromb Haemost 97:788-94
Kren, Betsy T; Yin, Wenxan; Key, Nigel S et al. (2007) Blood outgrowth endothelial cells as a vehicle for transgene expression of hepatocyte-secreted proteins via Sleeping Beauty. Endothelium 14:97-104
Perez-Pujol, Silvia; Marker, Paul H; Key, Nigel S (2007) Platelet microparticles are heterogeneous and highly dependent on the activation mechanism: studies using a new digital flow cytometer. Cytometry A 71:38-45
Zhang, Yan; Wroblewski, Matthew; Hertz, Marshall I et al. (2006) Analysis of chronic lung transplant rejection by MALDI-TOF profiles of bronchoalveolar lavage fluid. Proteomics 6:1001-10
Wang, Jian-Guo; Mahmud, Shawn A; Thompson, Jacob A et al. (2006) The principal eosinophil peroxidase product, HOSCN, is a uniquely potent phagocyte oxidant inducer of endothelial cell tissue factor activity: a potential mechanism for thrombosis in eosinophilic inflammatory states. Blood 107:558-65
Bach, Ronald R (2006) Tissue factor encryption. Arterioscler Thromb Vasc Biol 26:456-61
Marsik, C; Endler, G; Halama, T et al. (2006) Polymorphism in the tissue factor region is associated with basal but not endotoxin-induced tissue factor-mRNA levels in leukocytes. J Thromb Haemost 4:745-9
Ohlfest, John R; Frandsen, Joel L; Fritz, Sabine et al. (2005) Phenotypic correction and long-term expression of factor VIII in hemophilic mice by immunotolerization and nonviral gene transfer using the Sleeping Beauty transposon system. Blood 105:2691-8
Park, Chang Won; Kren, Betsy T; Largaespada, David A et al. (2005) DNA methylation of Sleeping Beauty with transposition into the mouse genome. Genes Cells 10:763-76

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