Abstract

The function is to provide Program Investigators with complete in vitro and in vivo gene delivery solutions to facilitatecompletion of their specific aims. This Core will:1. Achieve economy of scale and quality assurance through centralized generation of recombinant DNAand viral constructs by highly trained personnel.2. Provide technical expertise, consultation and training in PCR, RT-PCR, sequencing, recombinant DNAand protein expression and purification procedures.3. Establish and disseminate new genetic technologies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL066299-06
Application #
7217677
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2006-08-01
Project End
2011-07-31
Budget Start
2006-08-01
Budget End
2007-07-31
Support Year
6
Fiscal Year
2006
Total Cost
$167,556
Indirect Cost

  Institution

Name
University of South Alabama
Department
Type
DUNS #
172750234
City
Mobile
State
AL
Country
United States
Zip Code
36688

  Publications

Khozhukhar, Natalya; Spadafora, Domenico; Rodriguez, Yelitza et al. (2018) Elimination of Mitochondrial DNA from Mammalian Cells. Curr Protoc Cell Biol 78:20.11.1-20.11.14
Leavesley, Silas J; Sweat, Brenner; Abbott, Caitlyn et al. (2018) A theoretical-experimental methodology for assessing the sensitivity of biomedical spectral imaging platforms, assays, and analysis methods. J Biophotonics 11:
Lin, Mike T; Balczon, Ron; Pittet, Jean-Francois et al. (2018) Nosocomial Pneumonia Elicits an Endothelial Proteinopathy: Evidence for a Source of Neurotoxic Amyloids in Critically Ill Patients. Am J Respir Crit Care Med :
Parker, James C (2018) Mitochondrial damage pathways in ventilator induced lung injury (VILI): an update. J Lung Health Dis 2:18-22
Balczon, Ron; Morrow, K Adam; Zhou, Chun et al. (2017) Pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids. FASEB J 31:2785-2796
Shokolenko, Inna N; Alexeyev, Mikhail F (2017) Mitochondrial transcription in mammalian cells. Front Biosci (Landmark Ed) 22:835-853
Morrow, K Adam; Frank, Dara W; Balczon, Ron et al. (2017) The Pseudomonas aeruginosa Exoenzyme Y: A Promiscuous Nucleotidyl Cyclase Edema Factor and Virulence Determinant. Handb Exp Pharmacol 238:67-85
Shokolenko, Inna N; Wilson, Glenn L; Alexeyev, Mikhail F (2016) The ""fast"" and the ""slow"" modes of mitochondrial DNA degradation. Mitochondrial DNA A DNA Mapp Seq Anal 27:490-8
Kozhukhar, Natalya; Spadafora, Domenico; Fayzulin, Rafik et al. (2016) The efficiency of the translesion synthesis across abasic sites by mitochondrial DNA polymerase is low in mitochondria of 3T3 cells. Mitochondrial DNA A DNA Mapp Seq Anal 27:4390-4396
Leavesley, Silas J; Rich, Thomas C (2016) Overcoming limitations of FRET measurements. Cytometry A 89:325-7

Showing the most recent 10 out of 122 publications