Abstract

The function is to provide Program Investigators with complete in vitro and in vivo gene delivery solutions to facilitate? completion of their specific aims. This Core will:? 1. Achieve economy of scale and quality assurance through centralized generation of recombinant DNA? and viral constructs by highly trained personnel.? 2. Provide technical expertise, consultation and training in PCR, RT-PCR, sequencing, recombinant DNA? and protein expression and purification procedures.? 3. Establish and disseminate new genetic technologies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL066299-07
Application #
7515187
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2007-08-01
Budget End
2008-07-31
Support Year
7
Fiscal Year
2007
Total Cost
$180,816
Indirect Cost

  Institution

Name
University of South Alabama
Department
Type
DUNS #
172750234
City
Mobile
State
AL
Country
United States
Zip Code
36688

  Publications

Khozhukhar, Natalya; Spadafora, Domenico; Rodriguez, Yelitza et al. (2018) Elimination of Mitochondrial DNA from Mammalian Cells. Curr Protoc Cell Biol 78:20.11.1-20.11.14
Leavesley, Silas J; Sweat, Brenner; Abbott, Caitlyn et al. (2018) A theoretical-experimental methodology for assessing the sensitivity of biomedical spectral imaging platforms, assays, and analysis methods. J Biophotonics 11:
Lin, Mike T; Balczon, Ron; Pittet, Jean-Francois et al. (2018) Nosocomial Pneumonia Elicits an Endothelial Proteinopathy: Evidence for a Source of Neurotoxic Amyloids in Critically Ill Patients. Am J Respir Crit Care Med :
Parker, James C (2018) Mitochondrial damage pathways in ventilator induced lung injury (VILI): an update. J Lung Health Dis 2:18-22
Balczon, Ron; Morrow, K Adam; Zhou, Chun et al. (2017) Pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids. FASEB J 31:2785-2796
Shokolenko, Inna N; Alexeyev, Mikhail F (2017) Mitochondrial transcription in mammalian cells. Front Biosci (Landmark Ed) 22:835-853
Morrow, K Adam; Frank, Dara W; Balczon, Ron et al. (2017) The Pseudomonas aeruginosa Exoenzyme Y: A Promiscuous Nucleotidyl Cyclase Edema Factor and Virulence Determinant. Handb Exp Pharmacol 238:67-85
Blair, Leslie A; Haven, April K; Bauer, Natalie N (2016) Circulating microparticles in severe pulmonary arterial hypertension increase intercellular adhesion molecule-1 expression selectively in pulmonary artery endothelium. Respir Res 17:133
Spadafora, Domenico; Kozhukhar, Natalia; Alexeyev, Mikhail F (2016) Presequence-Independent Mitochondrial Import of DNA Ligase Facilitates Establishment of Cell Lines with Reduced mtDNA Copy Number. PLoS One 11:e0152705
Jian, Ming-Yuan; Liu, Yanping; Li, Qian et al. (2016) N-cadherin coordinates AMP kinase-mediated lung vascular repair. Am J Physiol Lung Cell Mol Physiol 310:L71-85

Showing the most recent 10 out of 122 publications