Core A, Microscopy, comprises valuable, electron and modern light microscopic tools that are made available as routine services to Program Project members. The services provided have been crucial for individual laboratory projects, and intra-programmatic research. Core A was established in the original application to meet the growing needs of the investigators in this area and has expanded in this renewal to include Dr. Farquhar as the core leader bringing her expertise as a microscopist due to the recent retirement of Dr. Feramisco. Electron microscopy facilities under her direction are located in the Center for Molecular Medicine and contain state of the art microscopes and specialized preparatory equipment. The core also includes Dr. Patel as a co-investigator who has expertise in light microscopy and has worked over ten years with Dr. Feramisco and the newly established School of Medicine (SOM) Microscopy Core which includes 2- photon confocal, spinning disk, super-resolution (purchase planned) and deconvolution systems. We propose in this application to fund two part-time research assistants to provide dedicated support to PPG members by 1) providing standardized training in the use of electron microscopy and 3-D fluorescence microscopes, 2) training individuals within this PPG to take full advantage of the enhanced scientific visualization software available in our group, and 3) providing hands-on help with the use of the many microscopes available. With increasing frequency, current PPG investigators are making use of these enhanced features, and the proposed Core A will facilitate the ability of PPG members to access these services in a timely and cost-efficient manner.

Public Health Relevance

Core A will provide basic and sophisticated tools including multiple microscopes, important training and, experimental design for PPG members. The goal will be to provide the best possible resources to visualize proteins and protein-protein interactions inside a cell specific to each project and experimental situation in a timely and cost effective manner.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
Project #
Application #
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Veterans Medical Research Fdn/San Diego
San Diego
United States
Zip Code
Suarez, Jorge; Cividini, Federico; Scott, Brian T et al. (2018) Restoring mitochondrial calcium uniporter expression in diabetic mouse heart improves mitochondrial calcium handling and cardiac function. J Biol Chem 293:8182-8195
Schilling, Jan M; Head, Brian P; Patel, Hemal H (2018) Caveolins as Regulators of Stress Adaptation. Mol Pharmacol 93:277-285
Giamouridis, Dimosthenis; Gao, Mei Hua; Lai, N Chin et al. (2018) Effects of Urocortin 2 Versus Urocortin 3 Gene Transfer on Left Ventricular Function and Glucose Disposal. JACC Basic Transl Sci 3:249-264
Penny, William F; Hammond, H Kirk (2017) Randomized Clinical Trials of Gene Transfer for Heart Failure with Reduced Ejection Fraction. Hum Gene Ther 28:378-384
Egawa, Junji; Schilling, Jan M; Cui, Weihua et al. (2017) Neuron-specific caveolin-1 overexpression improves motor function and preserves memory in mice subjected to brain trauma. FASEB J 31:3403-3411
Breen, Ellen C; Scadeng, Miriam; Lai, N Chin et al. (2017) Functional magnetic resonance imaging for in vivo quantification of pulmonary hypertension in the Sugen 5416/hypoxia mouse. Exp Physiol 102:347-353
Hastings, Randolph H; Montgrain, Philippe R; Quintana, Rick A et al. (2017) Lung carcinoma progression and survival versus amino- and carboxyl-parathyroid hormone-related protein expression. J Cancer Res Clin Oncol 143:1395-1407
Gao, Mei Hua; Lai, N Chin; Giamouridis, Dimosthenis et al. (2017) Cardiac-directed expression of a catalytically inactive adenylyl cyclase 6 protects the heart from sustained ?-adrenergic stimulation. PLoS One 12:e0181282
Miyanohara, Atsushi; Kamizato, Kota; Juhas, Stefan et al. (2016) Potent spinal parenchymal AAV9-mediated gene delivery by subpial injection in adult rats and pigs. Mol Ther Methods Clin Dev 3:16046
Hammond, H Kirk; Penny, William F; Traverse, Jay H et al. (2016) Intracoronary Gene Transfer of Adenylyl Cyclase 6 in Patients With Heart Failure: A Randomized Clinical Trial. JAMA Cardiol 1:163-71

Showing the most recent 10 out of 107 publications