This core is central for all transgenic and knockout experiments in this program project. Services that will beprovided for by the core will include the generation of transgenic, knock-in and knockout animals, genotypingand transporting animals to Dr. Lederer for Project 3. The core will also provide support for the individualprojects in the initial characterization of mouse models. The characterization of each mouse model willinclude careful analysis of the expression pattern of the transgene, and pathological and physiologicalchanges, which occur as a result of transgene expression. In the past 5 years of the grant period the coremanaged over 300 cages of mice per year, assisted in pathological analysis of all new transgenic andknockout mice and performed physiological measurements on the mice.The core will provide support for the PPG in the following areas:A. The preparation and maintenance of genetic mouse modelsB. Characterization of transgenic, knock-in and knock-out mice including preparation and analysis of tissuespecimens from animalsC. Baseline physiological measurements including hemodynamics and echocardiographyD. Physiological measurements on transgenic and knockout mice for drug testing.E. Non-invasive testing for VT in free ranging WT and transgenic miceF. Electrophysiological studies on the in vivo murine heart.G. Electrocardiogram and blood pressure recordings.
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| Zalk, Ran; Marks, Andrew R (2017) Ca2+ Release Channels Join the 'Resolution Revolution'. Trends Biochem Sci 42:543-555 |
| Williams, George S B; Boyman, Liron; Lederer, W Jonathan (2015) Mitochondrial calcium and the regulation of metabolism in the heart. J Mol Cell Cardiol 78:35-45 |
| Santulli, Gaetano; Pagano, Gennaro; Sardu, Celestino et al. (2015) Calcium release channel RyR2 regulates insulin release and glucose homeostasis. J Clin Invest 125:1968-78 |
| Drum, Benjamin M L; Santana, Luis F (2015) The long and winding road home: how junctin and triadin find their way to the junctional SR. J Mol Cell Cardiol 81:15-7 |
| Greiser, Maura; Kerfant, Benoît-Gilles; Williams, George S B et al. (2014) Tachycardia-induced silencing of subcellular Ca2+ signaling in atrial myocytes. J Clin Invest 124:4759-72 |
| Boyman, Liron; Chikando, Aristide C; Williams, George S B et al. (2014) Calcium movement in cardiac mitochondria. Biophys J 107:1289-301 |
| Ward, Christopher W; Prosser, Benjamin L; Lederer, W Jonathan (2014) Mechanical stretch-induced activation of ROS/RNS signaling in striated muscle. Antioxid Redox Signal 20:929-36 |
| Rullman, Eric; Andersson, Daniel C; Melin, Michael et al. (2013) Modifications of skeletal muscle ryanodine receptor type 1 and exercise intolerance in heart failure. J Heart Lung Transplant 32:925-9 |
| Mannella, Carmen A; Lederer, W Jonathan; Jafri, M Saleet (2013) The connection between inner membrane topology and mitochondrial function. J Mol Cell Cardiol 62:51-7 |
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