Abstract

This Core is designed to genotype individual mice used by all of the subprojects including mice with the following genes disrupted; epithelial nitric oxide synthase (eNOS); neuronal oxide synthase (nNOS); phagocytic oxidase p47/phox, NADPH oxidase; extracellular superoxide dismutase (EC-SOD) and the dopamine D5 receptor. Genotyping will be performed with real-time PCR using an ABI PRISM 77000 HT sequence detection system. The Core will also quantify mRNA expression for all of the subprojects in individual afferent, mesenteric and vasa recta arterioles by surgical microdissection under light microscope and/or laser microdissection and laser pressure catapulting, real-time PCR with multiplexing capabilities. In addition, Core C will determine protein expression by immunohistochemistry, Western blot, autoradiography and radioligand binding in renal and mesenteric microvessels, renal cortex and medulla. MRNA and protein expression will be determined for the following genes of interest including: eNOS, nNOS, components of NADPH oxidase including macrophage oxidase MOX-1, renal oxidase RENOX, hemeoxidase HO-1, HO-2, three isoforms of SOD, catalase, glutathione peroxidase, angiotensin AT1 and AT2 receptors and the dopamine D5 receptor. This core will also be responsible for keeping investigators informed of the latest molecular techniques applicable for each of the subprojects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
1P01HL068686-01
Application #
6540893
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
2001-09-30
Project End
2006-08-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
Georgetown University
Department
Type
DUNS #
049515844
City
Washington
State
DC
Country
United States
Zip Code
20057

  Publications

Asico, Laureano D; Cuevas, Santiago; Ma, Xiaobo et al. (2018) Nephron segment-specific gene expression using AAV vectors. Biochem Biophys Res Commun 497:19-24
Li, Lingli; Lai, En Yin; Luo, Zaiming et al. (2018) High Salt Enhances Reactive Oxygen Species and Angiotensin II Contractions of Glomerular Afferent Arterioles From Mice With Reduced Renal Mass. Hypertension 72:1208-1216
Schmidt, Marcel Oliver; Garman, Khalid Ammar; Lee, Yong Gu et al. (2018) The Role of Fibroblast Growth Factor-Binding Protein 1 in Skin Carcinogenesis and Inflammation. J Invest Dermatol 138:179-188
Tassi, Elena; Lai, En Yin; Li, Lingli et al. (2018) Blood Pressure Control by a Secreted FGFBP1 (Fibroblast Growth Factor-Binding Protein). Hypertension 71:160-167
Tassi, Elena; Garman, Khalid A; Schmidt, Marcel O et al. (2018) Fibroblast Growth Factor Binding Protein 3 (FGFBP3) impacts carbohydrate and lipid metabolism. Sci Rep 8:15973
Wang, Xiaoyan; Villar, Van Anthony; Tiu, Andrew et al. (2018) Dopamine D2 receptor upregulates leptin and IL-6 in adipocytes. J Lipid Res 59:607-614
Tiu, Andrew C; Bishop, Michael D; Asico, Laureano D et al. (2017) Primary Pediatric Hypertension: Current Understanding and Emerging Concepts. Curr Hypertens Rep 19:70
Li, Lingli; Lai, En Yin; Luo, Zaiming et al. (2017) Superoxide and hydrogen peroxide counterregulate myogenic contractions in renal afferent arterioles from a mouse model of chronic kidney disease. Kidney Int 92:625-633
Diao, Zhenyu; Asico, Laureano D; Villar, Van Anthony M et al. (2017) Increased renal oxidative stress in salt-sensitive human GRK4?486V transgenic mice. Free Radic Biol Med 106:80-90
Yang, Jian; Jose, Pedro A; Zeng, Chunyu (2017) Gastrointestinal-Renal Axis: Role in the Regulation of Blood Pressure. J Am Heart Assoc 6:

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