Abstract

Core C is the Molecular Biology and Biochemistry Core. This core is responsible for assisting eachof the subprojects in microdissection of isolated kidney segments, genotyping, gene silencing, andin quantitation of mRNA and protein expression. Genotyping will be performed with real-time PCRusing an ABI PRISM 7700 HT sequence detection system on mice in which the following geneshave been disrupted: the p22phox subunit of NADPH oxidase; extracellular superoxide dismutase(EC-SOD), intracellular superoxide dismutase (IC-SOD), manganese superoxide dismutase (Mn-SOD), angiotensin type 1a receptor (AT-1aR), dopamine type 5 receptor (D5R), fibroblast growthfactor type 2 (FGF-2) and type 7 (FGF-7) and the FGF binding protein (BP1) knockout andtransgene. Core C will also assist in silencing the following genes by siRNA: BP1, FGF-2 and FGF-7. In addition, Core C will assist in determining mRNA expression by real-time PCR, microarrayand in situ hybridization and in assaying protein expression by Western blot, immunohistochemistry,radioligand binding and autoradiography in order to assess siRNA efficacy and specificity. Thiscore will also be responsible for keeping investigators informed of the latest molecular techniquesapplicable for each of the subprojects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL068686-06
Application #
7218289
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2006-09-01
Project End
2011-08-31
Budget Start
2006-09-01
Budget End
2008-03-31
Support Year
6
Fiscal Year
2007
Total Cost
$198,116
Indirect Cost

  Institution

Name
Georgetown University
Department
Type
DUNS #
049515844
City
Washington
State
DC
Country
United States
Zip Code
20057

  Publications

Li, Lingli; Lai, En Yin; Luo, Zaiming et al. (2018) High Salt Enhances Reactive Oxygen Species and Angiotensin II Contractions of Glomerular Afferent Arterioles From Mice With Reduced Renal Mass. Hypertension 72:1208-1216
Schmidt, Marcel Oliver; Garman, Khalid Ammar; Lee, Yong Gu et al. (2018) The Role of Fibroblast Growth Factor-Binding Protein 1 in Skin Carcinogenesis and Inflammation. J Invest Dermatol 138:179-188
Tassi, Elena; Lai, En Yin; Li, Lingli et al. (2018) Blood Pressure Control by a Secreted FGFBP1 (Fibroblast Growth Factor-Binding Protein). Hypertension 71:160-167
Tassi, Elena; Garman, Khalid A; Schmidt, Marcel O et al. (2018) Fibroblast Growth Factor Binding Protein 3 (FGFBP3) impacts carbohydrate and lipid metabolism. Sci Rep 8:15973
Wang, Xiaoyan; Villar, Van Anthony; Tiu, Andrew et al. (2018) Dopamine D2 receptor upregulates leptin and IL-6 in adipocytes. J Lipid Res 59:607-614
Asico, Laureano D; Cuevas, Santiago; Ma, Xiaobo et al. (2018) Nephron segment-specific gene expression using AAV vectors. Biochem Biophys Res Commun 497:19-24
Tiu, Andrew C; Bishop, Michael D; Asico, Laureano D et al. (2017) Primary Pediatric Hypertension: Current Understanding and Emerging Concepts. Curr Hypertens Rep 19:70
Li, Lingli; Lai, En Yin; Luo, Zaiming et al. (2017) Superoxide and hydrogen peroxide counterregulate myogenic contractions in renal afferent arterioles from a mouse model of chronic kidney disease. Kidney Int 92:625-633
Diao, Zhenyu; Asico, Laureano D; Villar, Van Anthony M et al. (2017) Increased renal oxidative stress in salt-sensitive human GRK4?486V transgenic mice. Free Radic Biol Med 106:80-90
Yang, Jian; Jose, Pedro A; Zeng, Chunyu (2017) Gastrointestinal-Renal Axis: Role in the Regulation of Blood Pressure. J Am Heart Assoc 6:

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