Respiratory viral infections, particularly by human rhinoviruses (HRV), play important roles in inception and exacerbation of asthma. The goal of this PPG is to identify the HRV and host genetic determinants underlying the development of asthma in children and to determine the mechanisms by which HRV infections contribute to asthma pathogenesis. The Virology Core is established to provide this PPG with the capability to identify all common respiratory viruses and the individual serotype/strain of HRV associated with childhood asthma and to quantify the amount of HRV in the airways of subjects with wheezing. This Core will first examine comprehensively all nasal specimens with a new high-throughput, multi-target, sensitive and accurate viral assay called Respiratory MultiCode Assay that is capable of simultaneously detecting hundreds of strains of all common respiratory viruses belonging to 8 distinct groups (HRV, RSV, parainfluenza, influenza, metapneumovirus, enterovirus, coronavirus, and adenovirus) with a >4 hour completion time for a 96- well plate of samples. When a HRV is detected, its serotype/strain will be determined by a new molecular genetic typing method and HRV viral load in airway fluids will be measured with a new pan- HRV quantitative (q) PCR assay. In addition, this core will (1) provide uniform HRV stocks (including purified viruses, radiolabeled viruses, viruses with inactivated genome and high liter inoculum of 101 HRV serotypes), cDNA clones of selected serotypes and related materials and (2) perform standardized infectivity, qPCR assay and attachment assays of HRV in support of Projects l-lll, and (3) provide cultures of human bronchial epithelial cells for use in Projects II and III.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL070831-10
Application #
8375293
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
2013-09-25
Budget Start
2012-05-01
Budget End
2013-04-30
Support Year
10
Fiscal Year
2012
Total Cost
$418,907
Indirect Cost
$103,892
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
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Stein, Michelle M; Thompson, Emma E; Schoettler, Nathan et al. (2018) A decade of research on the 17q12-21 asthma locus: Piecing together the puzzle. J Allergy Clin Immunol 142:749-764.e3
Bønnelykke, Klaus; Coleman, Amaziah T; Evans, Michael D et al. (2018) Cadherin-related Family Member 3 Genetics and Rhinovirus C Respiratory Illnesses. Am J Respir Crit Care Med 197:589-594
Bashir, Hiba; Grindle, Kristine; Vrtis, Rose et al. (2018) Association of rhinovirus species with common cold and asthma symptoms and bacterial pathogens. J Allergy Clin Immunol 141:822-824.e9
Higano, Nara S; Bates, Alister J; Tkach, Jean A et al. (2018) Pre- and post-operative visualization of neonatal esophageal atresia/tracheoesophageal fistula via magnetic resonance imaging. J Pediatr Surg Case Rep 29:5-8
Higano, Nara S; Hahn, Andrew D; Tkach, Jean A et al. (2017) Retrospective respiratory self-gating and removal of bulk motion in pulmonary UTE MRI of neonates and adults. Magn Reson Med 77:1284-1295
Barkal, Layla J; Procknow, Clare L; Álvarez-García, Yasmín R et al. (2017) Microbial volatile communication in human organotypic lung models. Nat Commun 8:1770
Higano, Nara S; Fleck, Robert J; Spielberg, David R et al. (2017) Quantification of neonatal lung parenchymal density via ultrashort echo time MRI with comparison to CT. J Magn Reson Imaging 46:992-1000
Anderson, Halie M; Jackson, Daniel J (2017) Microbes, allergic sensitization, and the natural history of asthma. Curr Opin Allergy Clin Immunol 17:116-122
Kloepfer, Kirsten M; Sarsani, Vishal K; Poroyko, Valeriy et al. (2017) Community-acquired rhinovirus infection is associated with changes in the airway microbiome. J Allergy Clin Immunol 140:312-315.e8

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