The Microbiology and Biospecimen Core (""""""""MicroBio"""""""" Core) is established to provide viral and bacterial diagnostics, purified human rhinovirus (HRV) preparations, and cultured primary airway and nasal epithelial cells to each of the three proposed projects. The Core will provide support to Project II of this Program renewal application with the capability to identify all common respiratory viruses in samples of airway secretions and determine the individual species/type/strain of HRV. The quantity of selected bacterial pathogens {Moraxella catanrhalis, Hemophilus influenza and Streptococcus pneumoniae) in serial nasal lavage or sputum samples will be determined by multiplex qPCR, and partial sequencing will be used to determine whether bacteria that are sequentially detected represent a single strain (colonization) or serial infections with distinct strains. In addition, this Core will support the experiments of Projects l-lll by supplying (1) high-titer and uniform HRV virus stocks (2) antibodies targeting specific HRV proteins, (3) cultures of primary airway and nasal epithelial cells, and by (4) performing standardized HRV infectivity assays (plaque and TCID50) and quantitative RT-PCR and (5) infecting nasal epithelial cell cultures with HRV. In addition to fulfilling these aims, the MicroBio Core will also provide expertise for the use of these reagents and viral and bacterial diagnostic assays to COAST personnel and other collaborating investigators, and be available for consultation regaring molecular identification of respiratory viruses and bacteria, molecular biology of HRVs and the use of primary human airway epithelial cells.

Public Health Relevance

All three of the projects of the Childhood Origins of Asthma (COAST) Program proposal focus on how viral respiratory infections interact with genetic and environmental factors to cause inception of asthma and acute asthma exacerbations. The Microbiology and Biospecimen Core will provide top quality diagnostic testing, molecular reagents, and technical expertise to each of the COAST projects in support of this research goal.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL070831-11A1
Application #
8642809
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
2018-06-30
Budget Start
2013-09-26
Budget End
2014-06-30
Support Year
11
Fiscal Year
2013
Total Cost
$364,765
Indirect Cost
$113,672
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
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Stein, Michelle M; Thompson, Emma E; Schoettler, Nathan et al. (2018) A decade of research on the 17q12-21 asthma locus: Piecing together the puzzle. J Allergy Clin Immunol 142:749-764.e3
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Bashir, Hiba; Grindle, Kristine; Vrtis, Rose et al. (2018) Association of rhinovirus species with common cold and asthma symptoms and bacterial pathogens. J Allergy Clin Immunol 141:822-824.e9
Higano, Nara S; Bates, Alister J; Tkach, Jean A et al. (2018) Pre- and post-operative visualization of neonatal esophageal atresia/tracheoesophageal fistula via magnetic resonance imaging. J Pediatr Surg Case Rep 29:5-8
Jackson, Daniel J; Gern, James E; Lemanske Jr, Robert F (2017) Lessons learned from birth cohort studies conducted in diverse environments. J Allergy Clin Immunol 139:379-386
DeVries, Avery; Wlasiuk, Gabriela; Miller, Susan J et al. (2017) Epigenome-wide analysis links SMAD3 methylation at birth to asthma in children of asthmatic mothers. J Allergy Clin Immunol 140:534-542
Higano, Nara S; Hahn, Andrew D; Tkach, Jean A et al. (2017) Retrospective respiratory self-gating and removal of bulk motion in pulmonary UTE MRI of neonates and adults. Magn Reson Med 77:1284-1295
Barkal, Layla J; Procknow, Clare L; Álvarez-García, Yasmín R et al. (2017) Microbial volatile communication in human organotypic lung models. Nat Commun 8:1770
Higano, Nara S; Fleck, Robert J; Spielberg, David R et al. (2017) Quantification of neonatal lung parenchymal density via ultrashort echo time MRI with comparison to CT. J Magn Reson Imaging 46:992-1000

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