Approximately 200,000 people in the United States develop acute lung injury (ALI) and the acute respiratory distress syndrome (ARDS) annually. Despite research driven advances in therapy, 30%-40% of these patients will die. ALI and ARDS often manifest as a part of an inflammatory process characterized by acute failure of the alveolar-capillary membrane, which allows an exudate of plasma to flood the alveolar space. Influenza is the leading cause of death from an infectious cause in the United States and is an important cause of ARDS. Current influenza therapies inhibit viral proteins to prevent either replication or spreading of the virus while other strategies target host anti-viral responses However, the high genetic variability of the influenza virus results in variants that escape strain-specific adaptive immune responses or that are resistant to antiviral agents. Thus, targeting host cells proteins rather than viral proteins might prove to be a more effective strategy. Metabolism is an understudied area in acute lung injury and because viral metabolism requires highly conserved host proteins rather than viral proteins, targeting metabolic enzymes might provide a novel and effective strategy to prevent influenza-induced lung injury. We conducted a large scale metabolomics screen in primary human lung epithelial cells following infection with influenza A virus. These preliminary data indicate that influenza virus infection within hours causes the activation of catabolic pathways in the host cell reminiscent of autophagy and the upregulation of the glucose-dependent hexosamine biosynthetic pathway to generate the sialic acid N-acetylneuraminate?a metabolite required for efficient virus entry and exit from host lung epithelial cells. Furthermore, mice harboring 50% decrease in their maximal mitochondrial function recover quicker from influenza virus induced injury suggesting a causal role for changes in mitochondrial metabolism in regulating alveolar epithelial repair. The goal of this grant is to mechanistically determine the changes in metabolic pathways in epithelial cells that are essential for influenza virus induced lung injury. We propose three aims which will test whether (1) the glucose dependent hexosamine biosynthetic pathway is essential for influenza A virus production in lung epithelial cells; (2) autophagy within lungs epithelial cells is necessary for lung injury; (3) decreasing maximal mitochondrial capacity in lung epithelial cells accelerates recovery from lung injury.

Public Health Relevance

To date, the NHLBI ARDS network has identified only a single therapy that has favorably impacted survival in patients with ARDS. Thus, it is imperative to develop new therapeutic strategies. If our hypothesis that specific changes in metabolism play a causal role in the development of and recovery from lung injury then drugs targeting metabolism might be provide novel and effective therapies for these patients.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL071643-15
Application #
9735399
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Program Officer
Reineck, Lora A
Project Start
Project End
Budget Start
2019-07-01
Budget End
2020-06-30
Support Year
15
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Northwestern University at Chicago
Department
Type
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611
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