Abstract

The Biochemistry Core laboratory will provide biochemical assay services as well as histology and immunohistochemical analyses for the individual projects within the Program Project. Biochemical assays that will be performed include IL-6, IL-1beta, TNFalpha, TGFbeta, ET-1, microalbumin, norepinephrine, tissue total protein, plasma fibrinolytic activity, plasma renin, corticosterone, fibronectin, electrolytes, creatinine, 8- isoprostane, cGMP, 6-keto PGF(1alpha), PGE2, PGF(2alpha), TxB2 measurements in urine, plasma, and/or tissue extractsfrom mice and rats. Techniques will include radioimmunoassay, radioenzymatic assay, ELISA, and ion-selective electrodes. Histology and immunohistochemical analyses will provide hematoxylin & eosin (H&E), periodic acid-Schiff (PAS), Sirius red, blue trichrome, and specific immunohistochemical staining analyses for four of the projects in the Program Project. The core will also provide resources for development of additional assays and/or histological and immunohistological analyses as needed by individual investigators. The core will assure assay and histology quality control through validation procedures.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL074167-04
Application #
7433781
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2007-05-01
Project End
2009-04-30
Budget Start
2007-05-01
Budget End
2008-04-30
Support Year
4
Fiscal Year
2007
Total Cost
$295,858
Indirect Cost

  Institution

Name
Georgia Regents University
Department
Type
DUNS #
966668691
City
Augusta
State
GA
Country
United States
Zip Code
30912

  Publications

Guan, Z; Wang, F; Cui, X et al. (2018) Mechanisms of sphingosine-1-phosphate-mediated vasoconstriction of rat afferent arterioles. Acta Physiol (Oxf) 222:
Guan, Zhengrong; Singletary, Sean T; Cha, Haword et al. (2016) Pentosan polysulfate preserves renal microvascular P2X1 receptor reactivity and autoregulatory behavior in DOCA-salt hypertensive rats. Am J Physiol Renal Physiol 310:F456-65
Guan, Zhengrong; Singletary, Sean T; Cook, Anthony K et al. (2014) Sphingosine-1-phosphate evokes unique segment-specific vasoconstriction of the renal microvasculature. J Am Soc Nephrol 25:1774-85
Guan, Zhengrong; Fellner, Robert C; Van Beusecum, Justin et al. (2014) P2 receptors in renal autoregulation. Curr Vasc Pharmacol 12:818-28
Speed, Joshua S; Pollock, David M (2013) Endothelin, kidney disease, and hypertension. Hypertension 61:1142-5
Lobato, Nubia S; Filgueira, Fernando P; Prakash, Roshini et al. (2013) Reduced endothelium-dependent relaxation to anandamide in mesenteric arteries from young obese Zucker rats. PLoS One 8:e63449
Guan, Zhengrong; Giddens, Matthew I; Osmond, David A et al. (2013) Immunosuppression preserves renal autoregulatory function and microvascular P2X(1) receptor reactivity in ANG II-hypertensive rats. Am J Physiol Renal Physiol 304:F801-7
Lima, Victor V; Giachini, Fernanda R; Carneiro, Fernando S et al. (2011) O-GlcNAcylation contributes to the vascular effects of ET-1 via activation of the RhoA/Rho-kinase pathway. Cardiovasc Res 89:614-22
Choi, Hyehun; Tostes, Rita C; Webb, R Clinton (2011) S-nitrosylation Inhibits protein kinase C-mediated contraction in mouse aorta. J Cardiovasc Pharmacol 57:65-71
Inscho, Edward W; Cook, Anthony K; Clarke, Andrea et al. (2011) P2X1 receptor-mediated vasoconstriction of afferent arterioles in angiotensin II-infused hypertensive rats fed a high-salt diet. Hypertension 57:780-7

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