Abstract

Core D provides support to all individual projects in the program and provides methodology for the measurement of phospholipids, acylcarnitines and malonyl-CoA; isolation of mitochondria and enzyme activity measurements; preparation of tissue specimens and isolated mitochondria for morphological analysis.
The specific aims are: 1. Isolation of heart subsarcolemmal and interfibrillar mitochondria from control and microembolizationinduced heart failure dogs for oxidative phosphorylation measurement by Project 4. 2. Determine the activity of the electron transport chain enzymes. 3. Provide silica gel column chromatographic class separation of lipids and high performance liquid chromatographic analysis of phospholipid classes. 4. Provide quantitative and qualitative analysis of acylcarnitines, malonyl-CoA and phospholipids using high performance liquid chromatography/electrospray mass spectrometry. 5. Provide molecular weight and amino acid sequence determinations of modified proteins and/or peptides using high performance liquid chromatography/electrospray ionization mass spectrometry. 6. Provide additional analytical support procedures that the Core has the resources and expertise in even though the procedures are of limited scope in a particular project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
7P01HL074237-05
Application #
7462323
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2007-07-01
Budget End
2008-06-30
Support Year
5
Fiscal Year
2007
Total Cost
$365,431
Indirect Cost

  Institution

Name
University of Maryland Baltimore
Department
Type
DUNS #
188435911
City
Baltimore
State
MD
Country
United States
Zip Code
21201

  Publications

Sabbah, Hani N; Gupta, Ramesh C; Singh-Gupta, Vinita et al. (2018) Abnormalities of Mitochondrial Dynamics in the Failing Heart: Normalization Following Long-Term Therapy with Elamipretide. Cardiovasc Drugs Ther 32:319-328
Lanfear, David E; Gibbs, Joseph J; Li, Jia et al. (2017) Targeted Metabolomic Profiling of Plasma and Survival in Heart Failure Patients. JACC Heart Fail 5:823-832
Maruyama, Sonomi; Nakamura, Kazuto; Papanicolaou, Kyriakos N et al. (2016) Follistatin-like 1 promotes cardiac fibroblast activation and protects the heart from rupture. EMBO Mol Med 8:949-66
Sabbah, Hani N; Gupta, Ramesh C; Kohli, Smita et al. (2016) Chronic Therapy With Elamipretide (MTP-131), a Novel Mitochondria-Targeting Peptide, Improves Left Ventricular and Mitochondrial Function in Dogs With Advanced Heart Failure. Circ Heart Fail 9:e002206
Ardell, J L; Andresen, M C; Armour, J A et al. (2016) Translational neurocardiology: preclinical models and cardioneural integrative aspects. J Physiol 594:3877-909
Kop, Willem J; Galvao, Tatiana F; Synowski, Stephen J et al. (2015) Effects of environmental stress following myocardial infarction on behavioral measures and heart failure progression: The influence of isolated and group housing conditions. Physiol Behav 152:168-74
Roul, David; Recchia, Fabio A (2015) Metabolic alterations induce oxidative stress in diabetic and failing hearts: different pathways, same outcome. Antioxid Redox Signal 22:1502-14
Flori, Alessandra; Liserani, Matteo; Frijia, Francesca et al. (2015) Real-time cardiac metabolism assessed with hyperpolarized [1-(13) C]acetate in a large-animal model. Contrast Media Mol Imaging 10:194-202
Recchia, Fabio A (2015) Revascularization of hibernating myocardium: uneven reflorescence after the drought. J Am Coll Cardiol 65:698-700
Trappanese, Danielle M; Liu, Yuchuan; McCormick, Ryan C et al. (2015) Chronic ?1-adrenergic blockade enhances myocardial ?3-adrenergic coupling with nitric oxide-cGMP signaling in a canine model of chronic volume overload: new insight into mechanisms of cardiac benefit with selective ?1-blocker therapy. Basic Res Cardiol 110:456

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