Abstract

The mice (D1 and D3 receptor mutants and GRK4 wild type and A142V transgenes) required for project by Jose in this PPG will be bred and maintained by the Animal Core. Colonies to support adult mice of 8-14 weeks will be developed from initial breeders. Heterozygous (+/-) mice will be bred to provide homozygous (+/+) wild type, heterozygous (+/-) and homozygous (-/-) deleted mice in the same litter. The Animal Core has in place functioning breeding colonies and has a detailed plan that specifies the number of mice necessary to support the proposed research. The Core has the infrastructure in place to maintain breeding information in a database that catalogues ear tag identification and breeding history and numerous other parameters. The Animal Core will collect blood and/or tail tissue from weanling mice for genotyping in the Analytical Core. The Animal Core will also measure the conscious mean arterial blood pressure directly by radio-telemetry in a subset of each single-gene deleted or transgenic strain. The Animal Core will provide all technical support for all protocols involving animals and assure that the PPG is in compliance with all institutional regulations regarding animal breeding and experimental use.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL074940-05
Application #
7622667
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2008-04-01
Budget End
2009-03-31
Support Year
5
Fiscal Year
2008
Total Cost
$147,657
Indirect Cost

  Institution

Name
University of Virginia
Department
Type
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904

  Publications

Muntner, Paul; Whelton, Paul K; Woodward, Mark et al. (2018) A Comparison of the 2017 American College of Cardiology/American Heart Association Blood Pressure Guideline and the 2017 American Diabetes Association Diabetes and Hypertension Position Statement for U.S. Adults With Diabetes. Diabetes Care 41:2322-2329
Ye, Zhengmeng; Lu, Xi; Deng, Yi et al. (2018) In Utero Exposure to Fine Particulate Matter Causes Hypertension Due to Impaired Renal Dopamine D1 Receptor in Offspring. Cell Physiol Biochem 46:148-159
Yang, Yang; Chen, Caiyu; Fu, Chunjiang et al. (2018) Angiotensin II type 2 receptor inhibits expression and function of insulin receptor in rat renal proximal tubule cells. J Am Soc Hypertens 12:135-145
Li, Fengmin; Yang, Jian; Villar, Van Anthony M et al. (2018) Loss of renal SNX5 results in impaired IDE activity and insulin resistance in mice. Diabetologia 61:727-737
Wang, Xiaoyan; Villar, Van Anthony; Tiu, Andrew et al. (2018) Dopamine D2 receptor upregulates leptin and IL-6 in adipocytes. J Lipid Res 59:607-614
Luo, Hao; Chen, Caiyu; Guo, Li et al. (2018) Exposure to Maternal Diabetes Mellitus Causes Renal Dopamine D1 Receptor Dysfunction and Hypertension in Adult Rat Offspring. Hypertension 72:962-970
Wu, Gengze; Jose, Pedro A; Zeng, Chunyu (2018) Noncoding RNAs in the Regulatory Network of Hypertension. Hypertension 72:1047-1059
Asico, Laureano D; Cuevas, Santiago; Ma, Xiaobo et al. (2018) Nephron segment-specific gene expression using AAV vectors. Biochem Biophys Res Commun 497:19-24
Tiu, Andrew C; Bishop, Michael D; Asico, Laureano D et al. (2017) Primary Pediatric Hypertension: Current Understanding and Emerging Concepts. Curr Hypertens Rep 19:70
Diao, Zhenyu; Asico, Laureano D; Villar, Van Anthony M et al. (2017) Increased renal oxidative stress in salt-sensitive human GRK4?486V transgenic mice. Free Radic Biol Med 106:80-90

Showing the most recent 10 out of 123 publications