Abstract

The development of vascular heterogeneity is marked by regional changes in vascular morphology associated with alterations in cellular gene and protein expression. The ability to measure these changes at the single celt level is therefore crucial to investigate the mechanisms underlying the development of vascular heterogeneity. The goal of this Core is to provide the investigators of this proposal with a means of studying alterations in vascular development using state-of-the-art histochemical, immunohistochemical and gene expression analysis. All of the projects in this proposal will generate mice in which the function of a putative regulator of vascular development has been altered to study its role in vivo. This core will be responsible for processing the tissues of these animals and for their complete histologic analysis. This core is well-established and has generated a large percentage of the preliminary data in this proposal and contributed to numerous publications by the participating investigators. This core will provide a uniform, high level of quality for the proposed studies and the expertise to develop new methods that will be shared among the investigators.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL075215-04
Application #
7433796
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2007-06-01
Budget End
2008-05-31
Support Year
4
Fiscal Year
2007
Total Cost
$315,796
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Zhou, Zinan; Tang, Alan T; Wong, Weng-Yew et al. (2016) Cerebral cavernous malformations arise from endothelial gain of MEKK3-KLF2/4 signalling. Nature 532:122-6
Liu, Chunhong; Morishima, Masae; Jiang, Xiaoling et al. (2014) Engineered chromosome-based genetic mapping establishes a 3.7 Mb critical genomic region for Down syndrome-associated heart defects in mice. Hum Genet 133:743-53
Degenhardt, Karl R; Milewski, Rita C; Padmanabhan, Arun et al. (2010) Distinct enhancers at the Pax3 locus can function redundantly to regulate neural tube and neural crest expressions. Dev Biol 339:519-27
Rentschler, Stacey; Jain, Rajan; Epstein, Jonathan A (2010) Tissue-tissue interactions during morphogenesis of the outflow tract. Pediatr Cardiol 31:408-13
Stoller, Jason Z; Huang, Li; Tan, Cheryl C et al. (2010) Ash2l interacts with Tbx1 and is required during early embryogenesis. Exp Biol Med (Maywood) 235:569-76
Tian, Ying; Yuan, Lijun; Goss, Ashley M et al. (2010) Characterization and in vivo pharmacological rescue of a Wnt2-Gata6 pathway required for cardiac inflow tract development. Dev Cell 18:275-87
Jain, Rajan; Rentschler, Stacey; Epstein, Jonathan A (2010) Notch and cardiac outflow tract development. Ann N Y Acad Sci 1188:184-90
Degenhardt, Karl; Wright, Alexander C; Horng, Debra et al. (2010) Rapid 3D phenotyping of cardiovascular development in mouse embryos by micro-CT with iodine staining. Circ Cardiovasc Imaging 3:314-22
Hickey, Michele M; Richardson, Theresa; Wang, Tao et al. (2010) The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in mice. J Clin Invest 120:827-39
Cohen, Ethan David; Ihida-Stansbury, Kaori; Lu, Min Min et al. (2009) Wnt signaling regulates smooth muscle precursor development in the mouse lung via a tenascin C/PDGFR pathway. J Clin Invest 119:2538-49

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