Core C ? Proteomics Core Mass spectrometry (MS)-based proteomics is unequaled as a tool for studying complex biological systems and diseases in the post-genomic era. In the past decade, Drs. Gygi and Xiao have worked together and established close collaborations with the PIs of this PPG (Drs. Lefkowitz, Rockman, Stamler and Koch) to take full advantage of MS-based proteomics as a powerful tool to study ?-adrenergic receptor and angiotensin II type I receptor signaling. These collaborations form the basis for the Proteomics Core C, which will continue to provide cutting-edge, high-throughput MS technologies to the investigators of this PPG. Four major functions of the Proteomics Core are: 1) To serve as a proteomics resource for the three projects of this PPG, offering state-of-the-art proteomics service and consultation. The Core will provide cutting-edge MS-based proteomics technologies for both single protein characterization and large-scale, high-throughput MS-based proteomics analysis to support the cardiovascular GPCR research in this PPG. With these services, the Core will function as a ?discovery engine? in order to intensify the innovation efforts of the overall research program. 2) To support translational research in this PPG by conducting proteomic studies of heart samples from different sources. 3) To provide MS-based structural tools, such as Hydrogen-Deuterium Exchange MS (HDXMS), to study protein conformations and dynamics. 4) To continue to educate investigators involved in this PPG in the field of proteomics research, thereby disseminating this powerful technology. Three major services that the Proteomics Core will provide to this PPG are: 1) Basic MS-based proteomics services for protein identification and protein PTM analysis (such as phosphorylation, S-nitrosylation, ubiquitination, glycosylation, and prolyl hydroxylation, etc.). 2) Advanced large-scale high-throughput MS-based proteomics services (such as qualitative and quantitative interactome and phosphoproteome analysis). 3) MS services for studying protein conformation and conformational dynamics (such as HDXMS)

Public Health Relevance

Core C ? Proteomics Core Mass spectrometry (MS)-based proteomics is unequaled as a tool for studying complex biological systems and diseases in the post-genomic era. In the past decade, Drs. Gygi and Xiao have worked together and established close collaborations with the PIs of this PPG (Drs. Lefkowitz, Rockman, Stamler and Koch) to take full advantage of MS-based proteomics as a powerful tool to study ?-adrenergic receptor and angiotensin II type I receptor signaling. These collaborations form the basis for the Proteomics Core C, which will continue to provide cutting-edge, high-throughput MS technologies to the investigators of this PPG.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL075443-11
Application #
8932312
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2015-08-01
Budget End
2016-05-31
Support Year
11
Fiscal Year
2015
Total Cost
$400,567
Indirect Cost
$120,981
Name
Duke University
Department
Type
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
Kim, Jihee; Grotegut, Chad A; Wisler, James W et al. (2018) ?-arrestin 1 regulates ?2-adrenergic receptor-mediated skeletal muscle hypertrophy and contractility. Skelet Muscle 8:39
Zhou, Hua-Lin; Stomberski, Colin T; Stamler, Jonathan S (2018) Cross Talk Between S-Nitrosylation and Phosphorylation Involving Kinases and Nitrosylases. Circ Res 122:1485-1487
de Lucia, Claudio; Gambino, Giuseppina; Petraglia, Laura et al. (2018) Long-Term Caloric Restriction Improves Cardiac Function, Remodeling, Adrenergic Responsiveness, and Sympathetic Innervation in a Model of Postischemic Heart Failure. Circ Heart Fail 11:e004153
Grisanti, Laurel A; Schumacher, Sarah M; Tilley, Douglas G et al. (2018) Designer Approaches for G Protein-Coupled Receptor Modulation for Cardiovascular Disease. JACC Basic Transl Sci 3:550-562
de Lucia, Claudio; Eguchi, Akito; Koch, Walter J (2018) New Insights in Cardiac ?-Adrenergic Signaling During Heart Failure and Aging. Front Pharmacol 9:904
Wang, Jialu; Hanada, Kenji; Gareri, Clarice et al. (2018) Mechanoactivation of the angiotensin II type 1 receptor induces ?-arrestin-biased signaling through G?i coupling. J Cell Biochem 119:3586-3597
Hayashi, Hiroki; Hess, Douglas T; Zhang, Rongli et al. (2018) S-Nitrosylation of ?-Arrestins Biases Receptor Signaling and Confers Ligand Independence. Mol Cell 70:473-487.e6
Rizza, Salvatore; Cardaci, Simone; Montagna, Costanza et al. (2018) S-nitrosylation drives cell senescence and aging in mammals by controlling mitochondrial dynamics and mitophagy. Proc Natl Acad Sci U S A 115:E3388-E3397
Cannavo, Alessandro; Koch, Walter J (2018) GRK2 as negative modulator of NO bioavailability: Implications for cardiovascular disease. Cell Signal 41:33-40
Wang, Jialu; Gareri, Clarice; Rockman, Howard A (2018) G-Protein-Coupled Receptors in Heart Disease. Circ Res 123:716-735

Showing the most recent 10 out of 167 publications