Abstract

The purpose of the Vector Development Core is the derivation and production of recombinant adenoviral vectors to support specific projects within the PPG on Endothelial Cell Phenotypes in Health and Disease. The vector systems embraced within this proposal include both adenoviral vectors already generated in Gene Therapy Center at University of Alabama at Birmingham (UAB) and elsewhere (mainly viral vectors containing reporter genes), as well as novel viral vectors which have to be derived. We propose that the vector requirements of these projects will best be served by a central facility oriented towards derivation and evaluation of a range of vector reagents. The availability of validated vectors will allow a more rapid execution of the hypothesis-driven studies embodied in the aforementioned research projects. In addition, the creation of a centralized facility versed in the art of vector production will allow a cost- effective means of derivation of high quality reagents.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL076540-02
Application #
7077700
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2005-06-01
Budget End
2006-05-31
Support Year
2
Fiscal Year
2005
Total Cost
$148,892
Indirect Cost

  Institution

Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02215

  Publications

Yuan, Lei; Chan, Gary C; Beeler, David et al. (2016) A role of stochastic phenotype switching in generating mosaic endothelial cell heterogeneity. Nat Commun 7:10160
Bai, A; Kokkotou, E; Zheng, Y et al. (2015) Role of acid sphingomyelinase bioactivity in human CD4+ T-cell activation and immune responses. Cell Death Dis 6:e1828
Bai, Aiping; Moss, Alan; Rothweiler, Sonja et al. (2015) NADH oxidase-dependent CD39 expression by CD8(+) T cells modulates interferon gamma responses via generation of adenosine. Nat Commun 6:8819
Bai, Aiping; Robson, Simon (2015) Beyond ecto-nucleotidase: CD39 defines human Th17 cells with CD161. Purinergic Signal 11:317-9
Yan, Matthew S; Marsden, Philip A (2015) Epigenetics in the Vascular Endothelium: Looking From a Different Perspective in the Epigenomics Era. Arterioscler Thromb Vasc Biol 35:2297-306
Okada, Yoshiaki; Funahashi, Nobuaki; Tanaka, Toru et al. (2014) Endothelial cell-specific expression of roundabout 4 is regulated by differential DNA methylation of the proximal promoter. Arterioscler Thromb Vasc Biol 34:1531-8
Turgeon, Paul J; Sukumar, Aravin N; Marsden, Philip A (2014) Epigenetics of Cardiovascular Disease - A New ""Beat"" in Coronary Artery Disease. Med Epigenet 2:37-52
Bai, Aiping; Moss, Alan; Kokkotou, Efi et al. (2014) CD39 and CD161 modulate Th17 responses in Crohn's disease. J Immunol 193:3366-77
Rowe, Glenn C; Raghuram, Srilatha; Jang, Cholsoon et al. (2014) PGC-1? induces SPP1 to activate macrophages and orchestrate functional angiogenesis in skeletal muscle. Circ Res 115:504-17
Aird, William C; Mosnier, Laurent O; Fairhurst, Rick M (2014) Plasmodium falciparum picks (on) EPCR. Blood 123:163-7

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